基质细胞衍生因子1对骨髓间充质干细胞迁移及皮肤创伤修复的影响  

Effects of stromal cell-derived factor-1 on bone marrow mesenchymal stem cells migration and skin wound repair

作　　者：丁越[1] 徐海龙[1] 宋丛笑[1] 孙晓菊[1] 

机构地区：[1]辽宁省人民医院,辽宁省沈阳市110016

出　　处：《中国组织工程研究》2015年第6期843-848,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省科学技术计划项目(2013225021)"恶性肿瘤转化医学研究中心及协同研究网络建设"~~

摘　　要：背景:基质细胞衍生因子1对骨髓间充质干细胞具有强烈的趋化作用,且基质细胞衍生因子1与骨髓间充质干细胞均能促进组织创伤愈合,然而有关两者与皮肤创伤愈合的研究文献报道较少。目的:观察基质细胞衍生因子1在皮肤创伤修复过程中对骨髓间充质干细胞定向迁移及皮肤创面愈合的影响。方法:选取30只SD大鼠随机分为5组,各组大鼠尾静脉注射PKH26标记的骨髓间充质干细胞,注射1周后于背部制作皮肤创伤模型,造模后于皮肤创伤处多点注射不同质量浓度的基质细胞衍生因子1(1,2,10,50μg/L)。注射14 d后观察并记录大鼠皮肤愈合情况,免疫荧光染色观察创面组织骨髓间充质干细胞数量、分布情况,苏木精-伊红染色观察创面组织病理变化,Western blot检测创面组织Ⅰ型胶原及Ⅲ型胶原蛋白表达情况。结果与结论:当基质细胞衍生因子1质量浓度为10μg/L时,骨髓间充质干细胞在皮肤创面的数量最多,创伤修复效果最好。同样基质细胞衍生因子1能够调节Ⅰ型胶原及Ⅲ型胶原在创面的表达,基质细胞衍生因子1质量浓度为10μg/L时,Ⅰ型胶原及Ⅲ型胶原表达最高。结果表明适宜质量浓度的基质细胞衍生因子1(10μg/L)能够更好地促进骨髓间充质干细胞迁移,从而促进皮肤创伤愈合。BACKGROUND: Stromal cell-derived factor-1 has a strong chemotaxis to bone marrow mesenchymal stem cells,and both of them can promote wound healing. However, there are less studies on their correlation with skin wound healing.OBJECTIVE: To investigate the effects of stromal cell-derived factor-1 on bone marrow mesenchymal stem cells migration and skin wound repair.METHODS: Thirty SD rats were divided into five groups at random. Bone marrow mesenchymal stem cells labeled with PKH-26 were injected into the rat caudal vein. After 1 week, skin wound models were established. Then,different concentrations(1, 2, 10, 50 μg/L) of stromal cell-derived factor-1 were injected via multi-points on the skin wound. The skin wound healing was observed and recorded at 14 days after injection. The number and distribution of bone marrow mesenchymal stem cells were observed by the fluorescent staining at different time points. The pathological changes of wound tissue were observed by hematoxylin-eosin staining. The expression of collagen I and collagen III were detected by western blot assay.RESULTS AND CONCLUSION: Stromal cell-derived factor-1 at 10 μg/L could induce the largest number of bone marrow mesenchymal stem cells to the skin wound and achieve the best repair results. Stromal cell-derived factor-1 could also regulate the expression of collagen I and collagen III in the wound, and when the concentrationof stromal cell-derived factor-1 was 10 μg/L, the expressions of collagen I and collagen II reached the peak. These findings indicate that the appropriate concentration of stromal cell-derived factor-1 is better to promote the migration of bone marrow mesenchymal stem cells, thereby contributing to skin wound repair.

关 键 词：骨髓 间质干细胞移植 皮肤 创伤和损伤 趋化因子CXCL12 受体 CXCR4 干细胞 骨髓干细胞 基质细胞衍生因子1 骨髓间充质干细胞 皮肤创伤修复 

分 类 号：R751[医药卫生—皮肤病学与性病学]