猪环曲病毒N基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of porcine torovirus N protein gene

作　　者：周桐枫 周璐[1] 周远成[1] 刘小琬 徐志文[1,2] 朱玲[1,2] 郭万柱[1,2] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医学报》2015年第6期845-850,共6页Chinese Journal of Veterinary Science

基　　金：教育部长江学者和创新团队发展计划资助项目(IRT13083);教育部新世纪优秀人才支持计划资助项目(NCET 11-1059)

摘　　要：为研究猪环曲病毒(PToV)N蛋白的生物信息学特征,运用RT-PCR技术扩增猪环曲病毒N基因,测序并对其进行生物信息学分析。结果成功获得了492bp的PToV N基因(GenBank登录号为KM036209)。经生物信息学分析,此序列编码163个氨基酸,理论等电点(pI)为12.14,理论分子质量为18 695.5u,不稳定系数为77.47;不同密码子对同一氨基酸选择上呈现一定的偏嗜性;最大疏水指数为1.589,最小疏水指数为-3.533;无信号肽和跨膜区;抗原表位主要位于N末端和C末端。预测其可能包含4个N-糖基化作用位点,2个蛋白激酶C磷酸化作用位点、1个酪蛋白激酶Ⅱ磷酸化作用位点、1个N-豆蔻酰化位点。进化树分析结果显示,KM036209与韩国株和上海株亲缘关系很接近,与荷兰及西班牙株不属于同一分支。Porcine torovirus N gene was cloned by RT-PCR and sequenced,then bioinformatic analysis was conducted to investigate the structure and function of the porcine torovirus N gene.In result,a 492 bp porcine torovirus N gene was obtained(GenBank accession number:KM036209).Bioinformatics analysis showed that the sequence encoded 163 aa.The isoelectric point,relative molecular quality and instability index of porcine torovirus N were 12.14,18 695.5uand 77.47,respectively.An amino acid had bias of codons chossing.The maximum and the minimum hydrophobicity were 1.589and-3.533,respectively.The protein had no signal peptide and transmembrane domain;antigenic epitope may be located in the N-terminal and C-terminal.Motif searching showed that N protein might have 4N-glycosylation sites,2protein kinase C phosphorlation sites,1casein kinaseⅡ phosphorylation sites,1 N-myristoylation sites.Evolutionary tree showed that KM036209 had close genetic relationship with a Korea and a ShangHai strain,which was on the different branch with a Netherlands and a Spain strain.

关 键 词：猪环曲病毒 N基因 克隆 生物信息学分析 

分 类 号：S852.65[农业科学—基础兽医学]