仔猪免疫前后外周血T细胞CD28表达的初步研究  

The change on expression of CD28 pre-and post-immunization in piglet

作　　者：卢会鹏 刘铮铸[1] 史秋梅[1] 张艳英[1] 高桂生[1] 高光平[1] 马增军[2] 郭秀玲 陆奇玉 

机构地区：[1]河北科技师范学院动物科技学院河北省预防兽医重点实验室,河北昌黎066600 [2]河北省昌黎县畜牧发展局,河北昌黎066600

出　　处：《中国兽医学报》2015年第6期877-880,共4页Chinese Journal of Veterinary Science

基　　金：农业部转基因生物新品种培育科技重大专项资助项目(2014ZX0800603B);河北省高等学校创新团队领军人才培育计划资助项目(LJRC004)

摘　　要：为了分析免疫仔猪外周血T淋巴细胞CD28表达特点,采集接种蓝耳病弱毒疫苗和猪瘟弱毒疫苗的仔猪前后3d外周血液,并分离外周血单核细胞,采用实时定量PCR法检测仔猪外周血中T细胞CD28基因的相对表达量。结果显示仔猪在接种蓝耳病、猪瘟疫苗前后,CD28的表达有明显的提高,猪免疫前后CD28表达变化与机体接受抗原刺激有关。接种疫苗后,CD28在细胞内的表达明显增高,但与免疫的疫苗种类没有直接关系。To study the expression characteristics of CD28 on T-lymphocyte in piglet.Peripheral blood were collected from piglet vaccinated with attenuated PRRS virus vaccine,classical swine fever virus vaccine at pre and post three days.The segregated peripheral blood monouclear cell were reversed transcription using primer of Oligo(dT),and the relative expression of CD28 in T cells of peripheral blood were detected by Real-time quantitative PCR.The expression of CD28 increased greatly after immune the pre-immune attenuated PRRS virus vaccine and classical swine fever virus vaccine.The expression of CD28 gene in piglet changed after immune associated with the body antigen stimulation.After vaccination,the expression of CD28 on cells increased obviously,but had no direct relevance with the types of vaccine immune.

关 键 词：CD28 T细胞 RQ-PCR 仔猪 

分 类 号：S858.28[农业科学—临床兽医学]