雪山鸡THRSPα基因5′-侧翼区SNPs与肌肉品质关联性  

The association between the SNPs of 5′-flanking region in THRSPαgene and muscular quality traits of Xueshan chickens

作　　者：万方[1] 郭晓敏[1] 马腾[1] 王洪志[1] 陈静[1] 徐璐[1] 李志腾[1] 程立力 张康宁 袁青妍 许盛海 常国斌 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏立华牧业有限公司,江苏常州213168

出　　处：《中国兽医学报》2015年第6期1022-1026,共5页Chinese Journal of Veterinary Science

基　　金：国家863计划资助项目(2011AA100305);江苏省农业江苏省农业科技支撑资助项目(BE2013392)

摘　　要：通过MALDI-TOF-MS技术对雪山鸡甲状腺激素应答蛋白Spot 14(THRSP)α基因5′-侧翼区候选SNPs位点进行扫描,对SNPs的存在与否进行进一步验证,并对其与肌肉品质进行关联分析。结果表明:雪山鸡THRSPα基因5′-侧翼区中存在G637A突变,得到3种基因型,G、A等位基因频率分别为0.567和0.433,适合性检验处于遗传平衡状态;不同基因型个体间在pH值、肉色和嫩度等常规肉品质指标并无显著差异;但GA型个体胸肌肌内脂肪含量显著高于GG和AA型的个体(P<0.05)。GA个体腿肌的C16∶0含量、C18∶1n9c含量、C18∶2n6c含量均显著高于GG和AA型的个体(P<0.05),GG和AA型个体之间差异不显著。初步可以认为THRSPα基因应是肌肉脂肪性状含量的候选标记。To study the association between single nucleotide polymorphisms(SNPs)of THRSPαgene and muscular quality traits of Xueshan chickens,the candidate SNPs sites were detected by MALDI-TOF-MS technology.The results showed that a G637 A mutation was found in THRSPαgene,and there were three genotypes.The gene frequency of G was 0.567 and that of A was0.433.The fitness test for this SNPs site was in Hardy-Weinberg equilibrium.The normal meat quality traits,such as pH value,meat color and tenderness have no difference between the three genotypes.Intramuscular fat contents in chest of GA individuals were significantly higher than those of GG and AA individuals.C16∶0,C18∶1n9cand C18∶2n6ccontents in leg muscle of GA individuals were significantly higher than those of GG and AA individuals,which preliminarily revealed that THRSPαgene should be a candidate marker associated with the intramuscular fatty traits.

关 键 词：鸡 THRSPΑ基因 SNPS 

分 类 号：S831[农业科学—畜牧学]