Myostatin基因的克隆及免疫载体构建  

Myostatin gene cloning and construction of immune carrier

作　　者：李傲楠 杨润军[1] 田静[2] 于海滨[1] 芦春艳[2] 张晓军[1] 姜平[1] 赵志辉[1] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062 [2]吉林大学农业实验基地,吉林长春130062

出　　处：《中国兽医学报》2015年第2期252-256,共5页Chinese Journal of Veterinary Science

基　　金：国家"863"计划资助项目(2013AA102505);国家自然科学基金资助项目(31372278)

摘　　要：肌肉生成抑制素基因(Myostatin,MSTN),又称GDF-8,是TGF-β超家族中GDF亚族的成员之一,其对肌肉的生长发育具有负调控作用,该基因的缺失或突变可以造成肌纤维数目的增多或直径的增大。本试验根据Gen Bank已发表的牛的MSTN基因序列,设计特异性引物,利用巢式PCR方法,扩增MSTN基因的编码区。为构建具有高免疫原性的免疫载体,将MSTN基因插入到HBs Ag基因S区的3'末端,得到HBs Ag与MSTN基因相连接的融合表达质粒。经酶切和测序鉴定表明,重组质粒p EF1α-DsRed-S-MSTN构建成功,为后续的细胞和动物实验奠定基础。Myostatin,short for MSTN,is also called as GDF-8 which is a member of TGF-β supper family.Myostatin is a kind of glycoprotein which is widely expressed in skeletal muscle. Its function and changes of expression may be regulated by the expression of target gene to alter the muscle. In this experiment,the special primers were designed according to Gen Bank published cattle MSTN gene sequence. Nested PCR technology was used to amplificated the coding region of MSTN gene. In order to build the immune carrier with high immunogenicity,MSTN gene was inserted into HBs Ag in 3’ end. Then the HBs Ag gene connected to MSTN gene fusion expression plasmid was obtained. Enzyme digestion and sequencing test showed that recombinant plasmid p EF1α-DsRed-S-MSTN was built successful which lays the foundation for subsequent cell and animal experiments.

关 键 词：肌生成抑制素 基因免疫 融合质粒 载体构建 

分 类 号：Q78[生物学—分子生物学]