磷霉素对犬尿道致病性大肠杆菌的体外抗菌活性  

Antibacterial activity of fosfmoycin against canine uropathogenic Escherichia coli isolates

作　　者：刘海侠[1] 刘晓强[1] 李引乾[1] 蒿采菊 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国兽医学报》2015年第2期305-309,共5页Chinese Journal of Veterinary Science

基　　金：西北农林科技大学博士科研启动基金资助项目(2013BSJJ012)

摘　　要：应用微量稀释法和E-test,测定磷霉素对不同耐药类型的犬尿道致病性大肠杆菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),同时测定磷霉素对大肠杆菌的时间杀菌曲线。结果表明,磷霉素对大肠杆菌,包括对其他类抗菌药物耐药的多重耐药菌(MDR)具有良好的抗菌活性,其MIC和MBC分别为0.25~4.00 mg/L和0.50~32.00 mg/L。时间-杀菌曲线法试验结果显示,随着磷霉素浓度的增加,杀菌效应也明显增加,呈现明显的浓度依赖的特性。本研究为磷霉素临床控制动物致病性大肠杆菌感染及防控耐药性的产生提供了实验依据。Microdilution method and E-test were used to determine the minimum inhibitory concentration( MIC) and minimum bactericidal concentration( MBC),and the time-kill curves of fosfomycin against uropathogenic Escherichia coli( E. coli) isolated from canine. The results showed that fosfomycin had high antimicrobial activities to E. coli,including the multidrug resistance( MDR) isolates. The MIC and MBC ranged from 0. 25 to 4. 00 mg / L,and from 0. 50 to 32. 00 mg / L,respectively. The results of time-kill curve indicated that the bactericidal activity obviously increased with the increased concentration of fosfomycin,and showed an obvious concentration-dependent time-kill curve against uropathogenic E. coli. The present study provided experimental references for controlling the infection and resistance of pathogenic E. coli isolated from animals.

关 键 词：磷霉素 大肠杆菌 MIC MBC 时间-杀菌曲线 

分 类 号：S858.292[农业科学—临床兽医学]