2细胞期胚胎采集时间对电融合方法体外制备牛四倍体胚胎效率的影响  

Effects of two-cell stage embryos collection time on production efficiency of bovine tetraploid embryos by electrofusion

作　　者：赵静[1] 王二旦 柴梦龙 贺明[1] 刘畅[1] 杨世宝 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]临江市动物卫生监督所,吉林临江134600

出　　处：《中国兽医学报》2015年第2期335-339,共5页Chinese Journal of Veterinary Science

基　　金：吉林省现代农业产业技术体系建设资助项目(201327)

摘　　要：通过检测牛早期胚胎分裂速率的时间分布,并将体外受精后0~34 h与26~30 h时间段内采集的2细胞期胚胎分别作为电融合对照组与电融合试验组。将对照组与试验组的2细胞期胚胎分别用于电融合,并将电融合后的胚胎继续体外培养至囊胚期,检测并对比分析2组胚胎的融合率、分裂率、囊胚率和四倍体制备率。结果显示,牛2细胞期胚胎在体外受精后28~30 h其内分裂速率达到最高峰(18.9%)。电融合对照组的融合率、分裂率略高于试验组胚胎(85.2%vs.84.2%,82.2%vs.80.9%),试验组的囊胚率略高于电融合对照组(45.4%vs.44.8%),但差异均不显著。而电融合试验组的四倍体制备率显著高于电融合对照组(28.3%vs.14.5%)。结果表明,牛2细胞期胚胎的采集时间对电融合方法体外制备牛四倍体胚胎的效率有显著影响。The early embryonic cleavage rate was detected after IVF,and then established electrofusion control group( 0-34 h) and experimental group( 26-30 h) according to the 2-cell stage embryos which selected form different time quantum. Followed with electrofusion,preimplantation development of the fused embryos and the tetraploid rates were assessed between 2 groups. The results were as followed: the highest cleavage speed was achieved by 30 h after IVF( 18. 9%). The fused rate( 85. 2% vs. 84. 2%) and cleavage rate( 82. 2% vs. 80. 9%) of electrofusion control group were higher than experimental groups; meanwhile,the blastocyst rate( 45. 4% vs. 44. 8%) was lower than experimental group. Tetraploid production rates of electrofusion experimental group was significantly higher than electrofusion control group and experimental group( 28. 3% vs. 14. 5%). In conclusion,the 2-cell stage embryos collection time could significantly improve the production efficiency of bovine tetraploid by electrofusion.

关 键 词：四倍体胚胎 电融合 细胞周期 牛 

分 类 号：S814.8[农业科学—畜牧学]