多黏菌素E对鸡源性大肠杆菌和沙门菌的PAE及其机制初探  

The PAE of colistin on chicken-derived E.coli and S.pullorum

作　　者：张玲[1,2] 李继昌[1] 许薇[2] 孙媛[2] 王金莉[2] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030 [2]辽宁医学院畜牧兽医学院,辽宁锦州121001

出　　处：《中国兽医学报》2015年第4期585-591,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31472240);黑龙江省自然科学基金资助项目(C201424)

摘　　要：从发病鸡的粪便中分离出具有多重耐药性的大肠杆菌O18和鸡白痢沙门菌O2,检测多黏菌素E的最小抑菌浓度(MIC)和抗生素后效应(PAE),测定多黏菌素E接触过的细菌各组乳酸脱氢酶(LDH)和γ-谷氨酰基转移酶(γ-GT)活性变化、电镜观察细胞膜形态、巨噬细胞吞噬细菌数量和细菌对Caco-2的黏附力。多黏菌素E作用后,菌液中LDH和γ-GT活性显著性增高(P<0.01),细菌胞膜出现破损或缺失,细菌形态改变,被巨噬细胞吞噬的菌量明显增多(P<0.01),细菌对Caco-2的黏附力显著下降(P<0.01)。多黏菌素E对鸡源性多重耐药菌株存在PAE,推测其产生机制为破坏细菌细胞膜,改变细菌形态,促进巨噬细胞对细菌的吞噬能力增强,降低细菌对细胞黏附力。E.coli O18 and S.pullorum O2 were isolated from excrements of infected chickens.The minimum inhibitory concentration(MIC)and post antibiotic effect(PAE)of colistin were detected and the change of lactic dehydrogenase(LDH)and gamma-glutamyltransferase(γ-GT),membrane morphology,the number of macrophages engulfing bacteria and bacteria adhesion were investigated.Contrasted with control group,activity of LDH andγ-GT significantly increased in bacterium solution(P<0.01),bacterial cell membranes damaged,bacteria morphology changed,the number of bacteria engulfed by macrophages significantly increased(P<0.01),and the number of bacteria adhered to Caco-2cells significantly decreased(P<0.01)in colistin groups.Colistin has PAE on chicken-derived E.coli O18 and S.pullorum O2.The mechanism may be related with colistin destroying bacterial cell membranes,intensifying phagocytosis of macrophages and decreasing the number of bacteria adhesion.

关 键 词：多黏菌素E 鸡源性 大肠杆菌 鸡白痢沙门菌 抗生素后效应 

分 类 号：S859.7[农业科学—临床兽医学]