复合抗菌肽对断奶仔猪血清抗氧化功能的影响  

Effects of compound antibacterial peptide on antioxidant function of serum in weaned piglets

作　　者：但启雄 袁威[1] 李刚 胡洋 邓惠丹[1] 任志华[1] 田春雷[1] 金海涛[1] 邓又天[1] 邓俊良[1] 

机构地区：[1]四川农业大学动物医学学院动物疫病与人类健康重点实验室环境公害与动物疾病四川省高校重点实验室,四川雅安625014 [2]四川华德生物工程有限公司,四川成都610063

出　　处：《中国兽医学报》2015年第5期804-808,共5页Chinese Journal of Veterinary Science

基　　金：“长江学者和创新团队发展计划”创新团队基金资助项目(IRT0848)

摘　　要：为了探讨复合抗菌肽对断奶仔猪血清抗氧化功能的影响,选取90头26日龄、体质量相近(8.24±0.67)kg的杜长大三元杂交断奶仔猪,随机分成5组。对照组(Ⅰ组)饲喂基础日粮,Ⅱ组在基础日粮中添加黄芪多糖400mg/kg,Ⅲ~Ⅴ组在基础日粮中分别添加复合抗菌肽250、500、1 000mg/kg,分别检测第32、39、46和53日龄仔猪血清SOD、GSH-Px、T-AOC活力、抑制OH·能力和MDA含量。结果显示,各日龄点,Ⅱ、Ⅳ、Ⅴ组血清SOD活力显著高组组(P<0.01或0.05)(除Ⅱ组第32天),Ⅱ、Ⅳ、Ⅴ组血清MDA含量显著低于Ⅰ组(P<0.01或0.05)(除Ⅱ组第32天,Ⅳ组第39天),Ⅱ~Ⅴ组血清T-AOC和抑制OH·能力均显著高于Ⅰ组(P<0.01或0.05)。各试验指标随抗菌肽添加剂量的增加,效果更明显,以抗菌肽1 000mg/kg添加剂量最佳,优于黄芪多糖400mg/kg添加量(P<0.01或0.05)。由此表明,复合抗菌肽能够有效提高断奶仔猪血清抗氧化功能,增强仔猪抗断奶应激能力。To examine the effect of antibacterial peptides(ABP)and astragalus polysaccharide(AP)on the serum antioxidant function,90 healthy weaned piglets(26-day-old)were randomly divided into five groups based on weight(8.24±0.67)kg.The control group(Ⅰ)was fed basal diet,AP group(Ⅱ)was fed basal diet and AP 400mg/kg,ABP groupsⅢ-Ⅴ were fed basal diet with ABP at a dosage of 250 mg/kg,500 mg/kg,1 000 mg/kg,respectively.The serum antioxidant indices were determined at 32,39,46 and 53days of age.The results showed that the value of SOD in the groupsⅡ,Ⅳ,Ⅴ were markedly greater(P<0.01 or 0.05)than that higher of groupⅠ(except groupsⅡ,Ⅳ on 32d).The levels of serum GSH-Px in the groupsⅡ,Ⅳ,Ⅴ were significantly higher(P<0.01 or 0.05)than those of the groupⅠ(except groupⅡ on 32d).The contents of the serum MDA in the groupsⅡ,Ⅳ,Ⅴ were significantly lower(P<0.01 or 0.05)than those of groupⅠ(except groupⅡ on 32d,groupⅣ on 39d).The levels of serum T-AOC and inhibition of OH·capacity in the groupsⅡ-Ⅴwere significantly higher(P<0.01 or 0.05)than those of the groupⅠ.The antioxidant function of serum in the ABP groups was dose dependent,the optimum add dosage of ABP was found to be 1 000mg/kg,and was superior(P<0.01 or 0.05)to the 400mg/kg AP group.The present results indicated that ABP could effectively improve the serum antioxidant enzyme activity,and protect the cell from damage in weaned piglets.

关 键 词：复合抗菌肽 抗氧化指标 血清 断奶仔猪 黄芪多糖 

分 类 号：S828[农业科学—畜牧学]