花叶矢竹psaA基因克隆及功能分析  

The psa A Gene Cloning and Function Analysis of Pseudosasa japonica cv. Akebonosuji

作　　者：徐丽丽[1] 安苗苗[1] 姜可以[1] 许冰清[1] 杨海芸[1] 周明兵[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《竹子研究汇刊》2015年第1期41-48,共8页Journal of Bamboo Research

基　　金：国家自然科学基金(31170565;31270645;31470615);浙江省自然科学基金项目(LR12C16001;LY12C16002)

摘　　要：光系统I(PS I)主要分布在类囊体膜的非垛叠部分,由反应中心复合体和捕光复合体I(LHC I)等亚单位组成。PS I反应中心复合体的功能是将电子从PC传递给铁氧还蛋白,psa A所编码的蛋白Psa A是复合体重要组成部分。研究基于psa A高保守性,扩增出psa A全长序列,获得其编码区2516bp。对该序列及其推导出的氨基酸序列结构进行分析,将之与其他禾本科植物的psa A氨基酸序列进行同源性比较,并构建出基于psa A的系统进化树。通过定量RT-PCR调查了psa A基因在花叶矢竹叶色变异过程中表达丰度变化,分析其在花叶矢竹叶片发育过程中的作用,为进一步探讨花叶矢竹叶色变异的分子机理奠定一定的基础。The phtosystem I( PST I) of the plant chloroplast,which is composed of reactioncenter and light-harvesting complex I( LHC I) etc.,mainly distributes in the unfolding regions of thylakoid membrane. The electrons are transferred by the PS I reaction-center complex from the PC to ferredoxin. The psa A is an important part of the complex. In this study,based on the highly conserved psa A,the total length of the sequence of psa A in Pseudosasa japonica cv.akebonosuji were amplificated,with the ORF of 2516 bp. The encoded amino acid sequence was deduced,and then the phylogenetic tree of the psa A was constructed. Based on the quantitative RT-PCR,the expression abundance of the psa A was investigated during the leaf color variation of P. japonica cv. akebonosuji and its contribution was analyzed. This may be conducive to the further study on the molecular mechanism of leaf color variation of P. japonica cv. akebonosuji.

关 键 词：花叶矢竹 psaA基因 克隆 RT-PCR 功能分析 

分 类 号：Q943.2[生物学—植物学]