不同培养基对禾草内生真菌Epichloё生长与产孢的影响  

Effects of different medium on growth and sporulation of asexual stage of Epichloё endophytes

作　　者：柳莉[1] 李秀璋[1] 郭长辉[1] 李春杰[1] 

机构地区：[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2015年第6期859-869,共11页Pratacultural Science

基　　金：国家973计划课题(2014CB138702);国家自然科学基金项目(31372366)

摘　　要：本研究以分离自西藏早熟禾(Poa tibetica)、甘肃臭草(Melica przewalskvi)和麦宾草(Elymus tangutorum)的6个内生真菌菌株(分别为西藏早熟禾内生真菌Ep-003和Ep-004,甘肃臭草内生真菌Em-014和Em-016,麦宾草内生真菌Ee-001和Ee-003)为研究对象,分别在马铃薯葡萄糖琼脂(PDA)、麦秆煎液琼脂(WSA)、玉米粉琼脂(CMA)和水琼脂(WA)4种培养基上培养4周后,观测菌落生长速率、菌落形态特征、产孢量和孢子特性。结果表明,同一菌株在不同培养基上的生长速率一般为PDA和CMA上的最大,WSA和WA上最小;不同菌株在同一培养基上,西藏早熟禾的Ep-003菌株生长最快,在PDA上直径最大,为46.42 mm,麦宾草内生真菌次之,甘肃臭草的Em-014菌株生长最慢,在WA上直径最小,为7.07 mm;在4种培养基上,菌株的产孢量有显著差异(P<0.05),分别为Ep-003只在WA上产孢,其他5种菌株在WA和WSA培养基上产孢较多,在PDA和CMA上产孢较少;Em-016和Ep-004在4种培养基上的孢子长度无显著差异(P>0.05),Em-014在WSA上的孢子长最小,为3.77μm,其他菌株无显著差异(P>0.05),Ee-003在PDA上孢子长最长,为8.43μm,在其他3种培养基上无显著差异(P>0.05);Ep-004、Em-016和Ee-003在4种培养基上孢子梗长度均无显著差异(P>0.05),Em-014在WA上孢子梗最长,为16.74μm,在WSA上最短,为14.09μm,Ee-001在CMA上孢子梗最长,为18.59μm,在WSA上最短,为13.40μm。In the present study,six fungal strains of asexual stage of Epichloё endophytes were isolated from Poa tibetica( Ep-003,Ep-004),Melica przewalskyi( Em-014,Em-016) and Elymus tangutorum( Ee-001,Ee-003)and were cultured on different medium including potato dextrose agar( PDA),corn meal agar( CMA),wheat straw agar( WSA) and water agar( WA). The results showed that the same strain had different colonial morphologies on different medium which had the largest colonial diameter on PDA and CMA and the smallest colonial diameter on WSA and WA after four weeks growth on medium. For the performance of different strains on the same medium,the strain of Ep-003 was fastest growing with a culture diameter of 46. 42 mm on PDA whereas the strain of Em-014 was slowest growing with a culture diameter of 7. 07 mm on WA. There were significant difference between sporulation on different medium( P < 0. 05). Ep-003 only sporulated on WA and the other five isolates sporulated more on WSA and WA than on PDA and CMA. There was no significant differences between the spore length or width on different medium except with that Em-014 had the longest spore length of 3. 77 μm on WSA,and Ee-003 had the longest spore length of 8. 43 μm on PDA. In terms of the length of conidiophore,there was no significant differences between the length of Ep-004,Em-016 and Ee-003 on four different medium. Em-014 had the longest length of 16. 74 μm on WA and the shortest length of 14. 09 μm on WSA. However,Ee-001 had the longest length of 18.59 μm on CMA and the shortest length of 13. 40 μm on WSA.

关 键 词：共生真菌 分生孢子 生长速度 产孢特征 

分 类 号：S54[农业科学—作物学] Q939.11[生物学—微生物学]