农杆菌介导的朝鲜碱茅PuP5CS基因转化紫花苜蓿的研究  

Transformation of Puccinellia chinampoensis PuP5CS gene into alfalfa with Agrobacterium-mediated method

作　　者：徐博[1] 任伟[2] 王英哲[3] 孙启忠[3] 郭玮 娄玉杰[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林省农业科学院,吉林公主岭136100 [3]中国农业科学院草原研究所,内蒙古呼和浩特010010 [4]吉林省畜牧总站,吉林长春130062

出　　处：《草业科学》2015年第6期895-901,共7页Pratacultural Science

基　　金：吉林省科技厅青年科研基金(20140520177JH);吉教科合字[2015]第197号

摘　　要：为提高紫花苜蓿(Medicago sativa)的抗逆性,利用在朝鲜碱茅(Puccinellia chinampoensis)已克隆的Pu P5CS基因成功构建了p CAMBIA3300-Pu P5CS表达载体,以子叶为外植体,通过农杆菌介导共培养法转化紫花苜蓿"公农5号"(M.sativa cv.Gongnong No.5),并以2.0 mg·L-1的草铵膦进行筛选,抗性愈伤组织诱导率为22.4%,经草铵膦筛选的植株进行PCR检测和RT-PCR检测。结果显示,共获得11株转化植株,表明Pu P5CS基因已转入T0紫花苜蓿植株,且能够在RNA水平上正常表达。In order to cultivate resistance alfalfa( Medicago sativa),the plant expression vector p CAMBIA3300-Pu P5 CS was constructed and transformed into alfalfa from cotyledon via agrobacterium mediated. The transformed plants were selected with 2. 0 mg·L-1glufosinate ammonium and the ratio of transformation callus differentiation was 22. 4%. Eleven transformed plants were identified as positive in the gene expression by PCR and RT-PCR which suggested that the Pu P5 CS gene had been integrated into the genome of the regenerated plants.

关 键 词：PuP5CS 紫花苜蓿 遗传转化 农杆菌介导法 

分 类 号：Q943.2[生物学—植物学]