液泡膜Na^+/H^+逆向转运蛋白基因沉默对霸王叶气孔特征的影响  

Effects of tonoplast Na^+/ H^+ antiporters gene silence on leaf stomata characteristics of Zygophyllum xanthoxylum

作　　者：袁惠君[1,2] 马清[1] 未丽[1] 胡静[1] 王沛[1] 王锁民[1] 

机构地区：[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《草业科学》2015年第6期902-907,共6页Pratacultural Science

基　　金：973项目(2014CB138701);国家自然科学基金(31170431;31460629)

摘　　要：以霸王(Zygophyllum xanthoxylum)野生型植株(WT)、液泡膜Na+/H+逆向转运蛋白基因(Zx NHX)的RNA干扰(Zx NHX-RNAi)株系L2和L7为材料,分析了Zx NHX沉默后霸王叶下表皮气孔特征的变化情况。结果表明,50 mmol·L-1Na Cl处理下,Zx NHX-RNAi株系叶下表皮气孔开度达到峰值的时间比WT延迟了4 h;渗透胁迫下,L7气孔开度的峰值比WT显著下降12%。对照条件下,L7气孔大小显著低于WT,达到峰值的时间比WT延迟6h。无论是对照条件还是盐处理或渗透胁迫下,Zx NHX-RNAi株系叶组织含水量均显著低于WT,尤其是盐处理下,L7比WT低40%。可见,Zx NHX通过调控霸王叶的水分状况,进而影响气孔运动。The stomata characteristics of different Zygophyllum xanthoxylum lines including wild type( WT) and ZxNHX-silenced lines( L2 and L7) using post-transcriptional gene silencing were analyzed. Under 50 mmol·L-1Na Cl treatment,4 more hours was required for stomatal aperture of Zx NHX-silenced lines reached to peak value compared with WT. Under-0. 5 MPa osmotic stress treatment,the peak value of stomatal aperture in L7 lines significantly decreased by 12% compared with that of WT. Under control conditions,the stomatal size of L7 lines significantly reduced and 6 more hours was required for stomatal aperture reached to peak value compared with WT. Leaf tissue water contents in Zx NHX-silenced lines were always significantly lower than that in WT under all the treatments,especially under 50 mmol·L-1Na Cl treatment,leaf tissue water content of L7 lines was 40% less than that of WT. These results suggested Zx NHX regulated stomatal activities by controlling leaf water status of Z. xanthoxylum.

关 键 词：霸王 气孔 液泡膜Na+/H+逆向转运蛋白基因ZxNHX 基因沉默 

分 类 号：Q945[生物学—植物学]