高羊茅根际一种短体线虫的分离与鉴定  

Identification and separation of Pratylenchus from root of Festuca elata

作　　者：陈艳[1] 高利媛 马腾[2] 高文娜[2] 贺佳[1] 刘兴亮[2] 郭巍[1] 边勇[2] 

机构地区：[1]北京农学院,北京102206 [2]北京出入境检验检疫局,北京100026

出　　处：《草业科学》2015年第6期988-993,共6页Pratacultural Science

基　　金：国家质检总局科技计划项目"进境牧草种子真菌筛查技术研究初探"(2015IK021);北京出入境检验检疫局科技计划项目"北京地区进境植物繁殖材料隔离检疫模式适用与推行初探"(2013BK008);北京市农业技术试验示范项目(20140128)

摘　　要：高羊茅(Festuca elata)是草坪草中绿期最长的一个草种,在草坪建植中具有重要作用。一些病害随着高羊茅种植范围的不断扩大而逐渐频发,对草坪造成极大经济损失。本研究通过调查北京市高丽营镇高羊茅斑块状枯死的原因发现,导致高羊茅枯死的是高羊茅根际优势植物寄生线虫。采用贝尔曼漏斗法分离了侵染北京地区高羊茅上的线虫,结合唇环、受精囊、食道、V值等形态学鉴定方法,证明高羊茅草根际优势植物的寄生线虫均为一种短体线虫,确定为斯克里步纳短体线虫(Pratylenchus scribneri)。线虫ITS和28S序列的PCR扩增及Gen Bank序列比对后发现,其与国外发现的P.agilis及P.scribneri序列相似度为93%,亦确证该线虫为斯克里步纳短体线虫。Festuca elata is one of turfgrass species with longer green period and is very important in the construction of ecological environment. However,some diseases frequently occur and bring heavy economical loss to the lawn with the extended of tall fescue growing regions. In the present paper,an investigation about the reasons of dead patches in tall fescue lawn in Beijing's Gaoliying Town suggested that the predominant parasitic nematodes of tall fescue's rhizosphere were the primary cause of mortality. The parasitic nematodes isolated from tall fescue using the Behrman funnel method were short body nematodes with over 100 per 100 m L soil samples of population density.The parasitic nematodes were further identified as Pratylenchus scribneri based on morphology characteristics including esophagus,lip annulus,spermatheca,stylet length and V value. The internal transcribed spacer( ITS) region of r DNA was amplified from genomic DNA and sequenced. The sequence had 93% similarity with the species of P.agilis and P. scribneri in Genebank which confirmed the nematode was P. scribneri. To best of our knowledge,this is the first report that P. scribneri cause tall fescue patchy disease in China.

关 键 词：高羊茅 斯克里步纳短体线虫 分离鉴定 

分 类 号：S432.45[农业科学—植物病理学]