大菱鲆(Scophthalmus maximus)抗鳗弧菌(Vibrio anguillarum)性状的微卫星分子标记研究  

THE SCREENING OF MICROSATELLITES MOLECULAR MARKERS WITH REFERENCE TO ANTI-VIBRIO ANGUILLARUM OF TURBOT SCOPHTHALMUS MAXIMUS

作　　者：郭建丽[1] 田岳强 马爱军[1] 黄智慧[1] 商晓梅[1] 王新安[1] 杨志[3] 曲江波[3] 

机构地区：[1]中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室青岛市海水鱼类种子工程与生物技术重点实验室,青岛266071 [2]上海海洋大学,上海201306 [3]烟台开发区天源水产有限公司,烟台264003

出　　处：《海洋与湖沼》2015年第1期157-164,共8页Oceanologia Et Limnologia Sinica

基　　金：现代农业产业技术体系建设专项资金资助,CARS-50-G01号

摘　　要：采用微卫星分子标记技术分析验证了133对微卫星分子标记,以期为大菱鲆(Scophthalmus maximus)育种提供合适的抗鳗弧菌分子标记。实验鱼经鳗弧菌(Vibrio anguillarum)感染,分为非抗病组与抗病组。对两组鱼PCR扩增出的差异条带进行个体统计,再进行微卫星位点与抗鳗弧菌的相关性分析,并分析对比了两组鱼的遗传多样性。结果表明,微卫星位点Sma-USC108、Sma-USC141的等位基因片段分别在215bp和200bp与抗鳗弧菌性状的正相关性极显著,相关系数分别达到0.363和0.407(P<0.01);微卫星位点FF0911、Sma-USC62、Sma-USC279的等位基因片段分别在189bp、163bp和236bp与抗鳗弧菌性状的负相关性极显著,相关系数分别达到–0.377、–0.364和–0.363(P<0.01)。经过二次验证,最终确定Sma-USC141和FF0911两个微卫星位点可指导大菱鲆抗鳗弧菌的辅助育种。两组大菱鲆的群体遗传多样性分析对比表明:抗病群体与非抗病群体的遗传变异水平较低,两个群体的遗传多样性水平相当。We explored 133 pairs of microsatellite markers included in Gen Bank to obtain suitable molecular markers that closely associated with anti-Vibrio anguillarum for turbot(Scophthalmus maximus L.) breeding. The experimental turbot by intraperitoneal injection with Vibrio anguillarum were divided into two groups: susceptible group and resistant group. The differential bands in the two turbot groups were selected, from which the correlation between the upper anti-Vibrio anguillarum and the microsatellite loci were analyzed, and the genetic diversity compared. The results show that alleles of Sma-USC108 and Sma-USC141 at 215 bp and 200 bp respectively were extremely significantly correlated with anti-Vibrio anguillarum with the Pearson correlation value of 0.363 and 0.407. On the other hand, alleles of FF0911, Sma-USC62, and Sma-USC279 at 189 bp, 163 bp, and 236 bp, respectively, were extremely significantly negative-correlated with anti-Vibrio anguillarum, with the Pearson correlation values at –0.377, –0.364, and –0.363(P<0.01), respectively. We verified the above result with a second trial and determined that Sma-USC141 and FF0911 is useful for further molecular assistant selection of anti-Vibrio anguillarum turbot. Comparative analysis showed that they two groups were in low genetic variation at a same-level of genetic diversity.

关 键 词：大菱鲆 微卫星标记 鳗弧菌 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]