甘肃几种早熟禾内源激素水平及干旱适应性  

The Endogenous Hormone Level and Drought Adaptability of Four Kentucky Bluegrass Species in Gansu,China

作　　者：俞玲[1] 马晖玲[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点试验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《中国沙漠》2015年第1期182-188,共7页Journal of Desert Research

基　　金：国家自然科学基金项目(31160482)

摘　　要：以甘肃陇西、清水、秦州野生草地早熟禾为试验材料,商用品种午夜2号为对照,采用优化的HPLC法测定了干旱胁迫下草地早熟禾叶片中玉米素(ZT)、脱落酸(ABA)和吲哆乙酸(IAA)含量的变化。结果表明:干旱胁迫后,清水和秦州野生草地早熟禾植株萎蔫死亡,相比较之下陇西野生草地早熟禾和午夜2号却能够较好地适应干旱逆境。与此同时,供试材料的ZT含量均下降,下降幅度59.25%~66.02%;ABA和IAA含量均先上升后下降,但与对照相比ABA含量显著上升,上升幅度为23.63%~59.55%;IAA含量显著下降,下降幅度为48.34%~53.35%;此外,供试材料的ZT/IAA、ZT/ABA和(ZT+IAA)/ABA均下降,以减少生长量来主动适应干旱胁迫。在激素水平上评价材料的抗旱性大小为陇西野生草地早熟禾】午夜2号】秦州野生草地早熟禾】清水野生草地早熟禾。Some wild germplasm materials of Kentucky bluegrass collected from Gansu Province(Longxi,Qingshui,Qinzhou)were used as doner materials,and the MidnightⅡ,a commercial variety,was set as control.The content of abscisic acid(ABA),indole-3-acetic acid(IAA)and zeatin(ZT)in leaves of Kentucky bluegrass exposed to drought stress were determined by improved HPLC.The results showed that:The grass from Qingshui and Qinzhou wilted and died after the drought stress,however,the MidnightⅡ that from Longxi survived.ZT content of all the materials significantly decreased and the descent range was59.25%-66.02%;both ABA and IAA content increased firstly and then decreased,compared with the control,ABA content significantly increased and the rising range was 23.63%-59.55%;while IAA content significantly decreased,and the descent range was 48.34%-53.35%.In addition,the ZT/ABA、ZT/IAA and(ZT+IAA)/ABA decreased,suggesting that the grass could slow down growth to adapt to drought stress in initiative way.The drought resistance ability of the doner materials ranked as Longxi> MidnightⅡ >Qinzhou>Qingshui.It was done at the evaluation standard of hormones level.The study may provide reference evidences for the selection of excellent varieties and germplasm materials for turf establish in arid region.

关 键 词：干旱胁迫 早熟禾 内源激素 干旱适应性 相关性 

分 类 号：S688.4[农业科学—观赏园艺]