鸡KLF3基因的表达规律及其对脂肪细胞分化的影响研究  

Expression Pattern of Chicken Krüppel-like Factor 3 Gene and Its Effect on Adipocyte Differentiation

作　　者：王海霞[1] 张志威[1,2] 贺綦[1] 王宁[1] 王宇祥[1] 曹志平[1] 李辉[1] 

机构地区：[1]东北农业大学动物科学技术学院 农业部鸡遗传育种重点实验室 黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,哈尔滨150030 [2]石河子大学医学院,石河子832000

出　　处：《畜牧兽医学报》2015年第1期26-31,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家高技术研究发展计划(863计划)(2013AA102501);国家肉鸡产业技术体系建设项目(CARS-42);黑龙江省高等学校科技创新团队建设项目(2010td02)

摘　　要：为研究鸡KLF3(Gallus gallus Krüppel-like factor 3,gKLF3)基因的表达规律及其对脂肪细胞分化的影响,利用qRT-PCR检测了其在肉鸡脂肪组织和脂肪细胞中的表达特点,并利用基因过表达技术研究gKLF3基因对脂肪细胞分化的影响。结果显示,gKLF3在2~10周龄肉鸡腹部脂肪组织中持续表达,10周龄高脂肉鸡腹部脂肪组织gKLF3表达量显著高于低脂肉鸡(P<0.05);gKLF3在鸡成熟脂肪细胞的表达量低于前脂肪细胞(P<0.01);体外培养的鸡前脂肪细胞经油酸诱导分化后,gKLF3基因表达量均有低于对照组的趋势;过表达gKLF3基因有抑制脂肪细胞分化,以及抑制C/EBPα、FAS基因表达(P<0.01)的趋势。研究结果表明,gKLF3基因在鸡腹部脂肪组织生长发育过程中发挥重要作用,其可能是通过抑制C/EBPα、FAS基因表达进而抑制脂肪细胞分化实现的。This experiment was conducted to study the expression pattern of Krüppel-like factor 3(Gallus gallus KLF3,gKLF3)gene,and its effect on adipocyte differentiation.The expression pattern of gKLF3 in the adipose tissue and adipocytes of broilers were analyzed by using qRTPCR.The effect of gKLF3 on adipocyte differentiation was analyzed by over-expression technology.The result showed that gKLF3 was consecutively expressed during the chicken adipose tissue development from 2-to 10-week-old.At 10-week-old,the gKLF3 expression in the abdominal fat tissues was significantly higher in fat line chicken than that in lean line chicken(P<0.05).Additionally,the significantly higher gKLF3 expression level was observed in preadipocytes in contrast to that in mature adipocytes(P<0.01).The expression levels of gKLF3 in preadipocytes induced by oleate were lower than that in control.Moreover,over-expression of gKLF3 inhibited adipocyte differentiation and down-regula-ted expression of FASand C/EBPα(P<0.01).The result indicate that gKLF3 play an important role in the growth and development of chicken abdominal adipose tissue,and might restrain adipocyte differentiation by inhibiting the expression of C/EBPαand FAS.

关 键 词：鸡 KLF3基因 脂肪细胞分化 基因表达 

分 类 号：S831[农业科学—畜牧学]