鸡肝细胞中L-BABP基因表达对脂类代谢基因及甘油三酯和总胆固醇的影响  

The Effect of L BABP Gene Expression on the Lipid Metabolism Genes and Cholesterol and Triglyceride in Chicken Hepatocytes

作　　者：高广亮 张庆秋[1,2,3] 李辉 王启贵[1,2,3,4] 

机构地区：[1]农业部鸡遗传育种重点实验室,哈尔滨150030 [2]黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,哈尔滨150030 [3]东北农业大学动物科学技术学院,哈尔滨150030 [4]重庆市畜牧科学院,重庆402460

出　　处：《畜牧兽医学报》2015年第1期32-40,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30771542);现代农业产业技术体系建设项目(CARS-42);黑龙江省高等学校科技创新团队建设项目(2010td02)

摘　　要：本研究以鸡原代肝细胞为试验材料,探讨鸡L-BABP基因对脂类代谢相关基因及生化指标(总胆固醇、甘油三酯、低密度脂蛋白和高密脂蛋白)的影响。利用RNAi和过表达技术下调和上调该基因的表达,分别在干扰和过表达24、36、48、60和72h时检测鸡肝细胞中脂类代谢相关基因的表达量变化及细胞培养基中脂类代谢相关生化指标的变化。结果显示,鸡L-BABP基因表达量的变化显著或极显著影响APOB、APOAΙ和PERILIPIN基因表达,且显著地改变细胞培养基中的甘油三酯和总胆固醇的含量。基于以上结果,本研究推测鸡肝细胞中LBABP基因可能参与禽类脂肪沉积、总胆固醇代谢和脂解过程。This study was designed to investigate the role of L-BABP gene in lipid metabolism of hepatocytes and the effect of L-BABPgene on the genes related to lipid metabolism in chicken.LBABPgene expression was downregulated by siRNA interference and upregulated via overexpression plasmids method.The genes expression level in chicken hepatocytes were detected at 24,36,48,60 and 72hafter transfection with the siRNAs and overexpression plasmids of L-BABP.The results showed that APOAΙ,APOB,PERILIPIN gene expression levels,the amount of cholesterol and triglyceride in the medium are significantly affected by L-BABP gene expression changes.Therefore,L-BABP may involve in fat deposition,metabolism of total cholesterol and the process of lipolysis lipid metabolism.

关 键 词：鸡肝细胞 L-BABP基因 APOB基因 APOAI基因 Perilipin基因 总胆固醇 甘油三酯 

分 类 号：S852.2[农业科学—基础兽医学]