氨苄西林在鸡组织中的残留消除规律  

Ampicillin Residue Depletion in Chicken Tissues

作　　者：孙礼瑞 谢星[3] 谢恺舟[1,2] 张小杰[1,2] 王剑锋[1,2] 崔璐璐[1,2] 刘建宇[1,2] 张跟喜[1,2] 戴国俊[1,2] 王金玉[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,扬州225009 [3]南京农业大学动物医学院,南京210095

出　　处：《畜牧兽医学报》2015年第1期130-137,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家肉鸡产业技术体系专项(CARS-42-G23);“十二五”国家科技支撑计划(2014BAD13B02);江苏高校优势学科建设工程项目;扬州大学“新世纪人才工程”项目

摘　　要：旨在研究氨苄西林在鸡组织中残留消除规律。鸡组织样品经磷酸二氢钠溶液提取,乙腈去蛋白质,正己烷去脂肪,饱和二氯甲烷萃取,上清液经甲醛在酸性条件下沸水浴衍生化后,在激发波长327nm、发射波长409nm处用高效液相色谱荧光检测器检测。结果显示:该方法测定鸡组织中氨苄西林的检测限为3.5μg·kg-1(S/N=3)、定量限为10.0μg·kg-1(S/N=10)。氨苄西林在鸡组织样品中平均回收率在75.31%~85.87%,变异系数均低于10.20%。各试验组京海黄鸡分别按体重以120、240mg·(kg·d)-1剂量内服氨苄西林,每天1次,连续7d给药后,休药4h(零休药期),各组织中氨苄西林的残留量最高,并在休药后第3天迅速降低。休药第5天后所有组织中氨苄西林残留量均低于最高残留限量(50μg·kg-1),休药第9天各组织中氨苄西林残留量均低于检测限。休药后相同时间点鸡肌肉中药物残留量最低,肝中的药物残留量最高;氨苄西林在肾中残留比肌肉、肝消除缓慢且消除时间长。氨苄西林在鸡肌肉、肝和肾中的残留量均与给药剂量呈正相关。根据WT1.4软件按95%置信区间计算所得,建议黄羽肉鸡按体重以120、240mg·(kg·d)-1剂量给药,每天1次,连续7d后,其休药期(WT)分别为4和5d。A study on ampicillin(AMP)residue depletion was conducted in chicken tissues.AMP was extracted from chicken tissues with sodium dihydrogen phosphate solution.After being deproteinizated with acetonitrile and degreased in n-hexane saturated,the analyte was extracted by saturated dichloromethane.The supernatant was reacted with formaldehyde under acidic and boiling conditions.Finally,AMP was determinated by high performance liquid chromatography(HPLC)with fluorescence detector.The excitation wavelength was set at 327 nm and emission wavelength was set at 409 nm.The average recovery in chicken tissue was 75.31%-85.87% with coefficients of variation(CV)lower than 10.20%.The limit of detection(LOD)was 3.5μg·kg-1(S/N=3)and the limit of quantitation(LOQ)was 10.0μg·kg-1(S/N=10).After the chickens were orally administered successively AMP of 120mg·kg-1 and 240mg·kg-1 of body weight one time every day for 7days,the maximum residues of AMP in chicken tissues were detected at 4hafter final administration.Concentrations of AMP fell sharply at the 3th withdrawal day,all lower than MRL(50μg·kg-1)at the 5th withdrawal day,and all lower than LOD(5.0μg·kg-1)at the 9th withdrawal day.At the same time definition,the concentration of AMP in muscle was lowest,but highest in liver.However,AMP in kidney needed more time for depletion.The residues of AMP in chicken tissues were all positively correlated with AMP orally administered doses.The concentration-time dates were analyzed by WT1.4software to get the withdrawal time(WT)with 95% upper tolerance limit,the WT advised in normal dose group was 4d,and in double dose group was 5d.

关 键 词：氨苄西林 高效液相色谱荧光检测法 残留消除 鸡组织 

分 类 号：S859.84[农业科学—临床兽医学]