芪鹿消白方对阿霉素肾病大鼠肾小球硬化的作用及其机制  

Effect of Qiluxiaobai decoction on glomerular sclerosis in adriamycin-induced nephropathic rats

作　　者：苏中昊[1] 叶进[1] 庞慧芳[1] 

机构地区：[1]上海中医药大学基础医学院,上海201203

出　　处：《中南大学学报（医学版）》2015年第1期83-89,共7页Journal of Central South University ：Medical Science

基　　金：2012上海中医药大学中医临床基础学科建设课题(12ZLJ07);2014上海中医药大学中青年骨干教师教学能力提升资助计划~~

摘　　要：目的:探讨芪鹿消白方(Qiluxiaobai,QLXB)对阿霉素致局灶节段性肾小球硬化(focal segmental glomerulosclerosis,FSGS)大鼠的影响。方法:大鼠采用随机数字表法分为4组:正常组、模型组、科素亚组、QLXB组。采用7.5 mg/kg分2次尾静脉注射阿霉素致大鼠肾脏FSGS。科素亚组予科素亚5.19 mg/(kg·d),QLXB组予QLXB134.4 mg/(kg·d)灌胃给药,正常组以生理盐水代替。观察8周。定期检测体质量、血肌酐、血尿素氮等生化指标;光镜下观察肾组织HE和PAS染色形态学变化。RT-PCR和Western印迹检测肾组织纤维化指标结缔组织生长因子(connective tissue growth factor,CTGF),纤维连接蛋白(fibronectin,FN),平滑肌肌动蛋白-α(smooth muscle actin-α,α-SMA)。结果:与正常组比较,模型组血肌酐、尿素氮、总胆固醇、三酰甘油水平升高(P<0.05),血清白蛋白明显降低(P<0.05);光镜下肾组织结构破坏;CTGF,FN,α-SMA m RNA和蛋白表达明显升高(P<0.05)。科素亚和QLXB能降低血肌酐、尿素氮、总胆固醇、三酰甘油水平,升高血清白蛋白水平(P<0.05)。QLXB减轻肾组织病理改变较科素亚明显,并能抑制肾组织CTGF,FN,α-SMA m RNA和蛋白表达的明显上调(P<0.05)。结论:QLXB能在一定程度上改善阿霉素肾病肾小球硬化病变,主要机制可能与抑制肾组织CTGF,FN,α-SMA m RNA和蛋白表达有关。Objective: To explore the effect of Qiluxiaobai(QLXB) decoction on rats with adriamycin(ADR)-induced focal segmental glomerular sclerosis(FSGS) nephropathy(ADN). Methods: Adriamycin was injected into tail vein at total dose of 7.5 mg/kg for twice per week. According to random number table, rats were divided into 4 groups: the control group, the ADN group, the Losartan group [intragastric, 5.19 mg/(kg·d)], and the QLXB group [intragastric, 134.40 mg/(kg·d)]. Eight weeks later, serum creatinine(SCr), blood urea nitrogen(BUN), serum cholesterol(CHO), serum triglycerides(TG) and albuminuria(ALB) were measured by routine biochemical methods. Pathological changes in the rat kidneys were observed under light microscopes. Connective tissue growth factor(CTGF), α-smooth muscle actin(α-SMA) and fibronectin(FN) m RNA and protein expression levels were measured by real-time PCR and Western blot, respectively. Results: In the ADN group, SCr, BUN, CHO, TG was increased(P<0.05) while ALB was decreased(P<0.05), ALB was decreased(P<0.05) compared to the control group. In the QLXB and Losartan group, SCr, BUN, CHO, TG and ALB was improved compared to the ADN group(P<0.05). CTGF, FN, α-SMA m RNA and protein expression was decreased in QLXB group compared to ADN group(P<0.05). Conclusion: QLXB could partly improve glomerular sclerosis in adriamycin-induced nephropathy, which was related to inhibition of CTGF, FN and α-SMA expression.

关 键 词：慢性肾病 蛋白尿 芪鹿消白方 阿霉素肾病 结缔组织生长因子 纤维连接蛋白 平滑肌肌动蛋白-α 

分 类 号：R285.5[医药卫生—中药学]