辣椒DBP/DIBP胁迫及其修复剂优化和机理研究  

Pepper Growth Influenced by and Formula Optimization and Mechanisms of Remediation for DBP/DIBP

作　　者：任旭琴[1,2] 高军[1,2] 陈伯清[1,2] 骆丹丹[1] 

机构地区：[1]淮阴工学院生命科学与化学工程学院,江苏淮安223003 [2]江苏省凹土资源利用重点实验室,江苏淮安223003

出　　处：《农业环境科学学报》2015年第6期1121-1126,共6页Journal of Agro-Environment Science

基　　金：江苏省农业科技支撑计划项目(BE2013376);江苏省自然科学基金项目(KB20131216);江苏省大学生实践创新计划项目(201311049055X)

摘　　要：在研究邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二异丁酯(DIBP)对辣椒生长胁迫效应的基础上,选择凹土、蜂窝煤渣、腐植酸组成混合修复剂,采用Box-Behnken试验设计构建回归模型,优化修复剂配方,进而探讨了修复机理。结果表明:土壤中DBP/DIBP含量达到20 mg·kg-1以上时,对辣椒生长和干物质积累有显著抑制作用;回归模型极显著(P<0.01),可用于分析预测DBP/DIBP的修复效果;修复剂最佳配方为15.91 g·kg-1凹土+16.40 g·kg-1蜂窝煤渣+1.67 g·kg-1腐植酸;施用修复剂后,土壤酶活性显著增强,根系的过氧化物酶(POD)和过氧化氢酶(CAT)活性显著提高,丙二醛(MDA)含量显著降低,根系活跃吸收面积增大,根系活力增强,辣椒干重增加20.91%。In the present study, the effects of dibutyl phthalate(DBP)and diisobutyl phthalate(DIBP)on pepper growth were studied, and the remediation formula for DBP/DIBP was also optimized using Box-Behnken experimental design and regression model. Meanwhile, the remediation mechanisms were discussed. The growth and dry weight of pepper were significantly inhibited by 20 mg·kg-1DBP/DIBP in soil.The regression model predicted the remediation effects of formula applied very well(P<0.01). The optimized formula was 15.91 g·kg-1 of attapulgite, 16.40 g·kg-1of honeycomb residue and 1.67 g·kg-1of humic acid. Applying optimized formula significantly increased urease, dehydrogenase, protease and saccharase activities in rhizospheric soil, and improved peroxidase(POD)and catalase(CAT) in pepper roots,but decreased MDA content as compared to the control. Under optimized formula, the area and activity of pepper roots were increased and the dry matter of pepper improved by 20.91%. The application of optimized formula effectively alleviated the negative effects of DBP/DIBP on pepper.

关 键 词：凹土 辣椒 邻苯二甲酸二丁酯 邻苯二甲酸二异丁酯 

分 类 号：S641.3[农业科学—蔬菜学]