猪Fosl2基因克隆、结构预测及其mRNA发育性表达变化  

Gene Cloning,Bioinformatics Analysis and Developmental Expression of Fosl2 Gene in Pig

作　　者：范兴兴[1] 李新建[1] 李改英[1] 郭吉利[1] 韩雪蕾[1] 吕刚[1] 任广志[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《畜牧兽医学报》2015年第2期211-218,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河南省生猪产业技术体系创新团队(S2012)

摘　　要：本试验旨在克隆猪Fosl2基因CDS序列并对其进行分析,研究该基因在猪的不同阶段各种组织(心、肝、脾、肺、肾、背最长肌、背部脂肪)中的发育性表达规律。以豫南黑猪为材料,利用RT-PCR技术克隆猪Fosl2基因CDS区域,利用生物信息学方法预测蛋白结构,应用qRT-PCR检测豫南黑猪7~180日龄上述组织中Fosl2基因的发育性表达。结果表明,猪Fosl2基因的CDS区为984bp,编码327个氨基酸,跨膜序列为17~33位氨基酸,该肽链没有信号肽,为跨膜非分泌型蛋白,在122~187位氨基酸的位置存在亮氨酸拉链结构域,核苷酸和氨基酸序列同其他种属间的保守性很高。Fosl2基因拥有广泛的组织表达谱,在心、肝、脾、肾、肺、背最长肌和背部脂肪的不同发育阶段均有不同程度的表达,而其在肺中的表达量均高于同时期其他组织内的表达量,推测猪Fosl2基因可能与肺部组织的发育有关;其在背部脂肪不同发育阶段的变化趋势表明其可能参与调控猪脂肪的生成进而影响瘦肉率。本研究结果将为进一步研究猪Fosl2结构及其多功能奠定基础。The study was conducted to clone and analyze CDS sequence of pig Fosl2 gene,and to investigate the developmental expression of Fosl2 in pig various tissues(heart,liver,spleen,lung,kidney,the longissimus muscle and backfat)at different stages.In this study,Yunan Black pig was used as experimental animal to clone the CDS region of Fosl2 gene by RT-PCR,the protein structure were predicted by bioinformatics,meanwhile,qRT-PCR was used to analyze developmental expression of Fosl2 gene in pig from 7-to 180-day-old.The results showed that the CDS of pig Fosl2 gene was 984 bp,encoding 327 amino acids.It had no signal peptide in peptide chain,but had a transmembrane domain and a basic-domain leucine-zipper,which were from 17 to 33amino acids and from 122 to 187amino acids,respectively.The protein of pig Fosl2 had high conservatism among mammal,and it was transmembrane and non-secretory protein.The Fosl2 was widely expressed in tissues,the expression of Fosl2 in lung was higher than other tissues at the same stage,it was deduced that pig Fosl2 might be related to the process of lung development;the variation trend in backfat showed that Fosl2 might had the function enhancing adipogenesis and then regulate lean meat percentage.Therefore,the results of this study may lay foundation for furtherresearch on the structure and the role of Fosl2 gene in physiogenesis in pig.

关 键 词：猪 Fosl2 克隆 序列分析 发育性表达 

分 类 号：S828[农业科学—畜牧学]