小型猪复合麻醉颉颃剂对大鼠各脑区p-p38蛋白及c-myc mRNA表达的影响  

Effect of the Antagonist for the Combined Anaesthetic for Miniature Pigs on p-p38 Protein and c-myc Gene in Brain Regions of Rats

作　　者：李妍[1] 侯金龙[1] 牛栋梁[1] 姜胜[1] 时静[1] 范宏刚[1] 王洪斌[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《畜牧兽医学报》2015年第2期317-322,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31272617;31001092)

摘　　要：旨在研究小型猪复合麻醉颉颃剂对大鼠不同脑区p-p38蛋白及c-myc mRNA表达的影响,探讨小型猪复合麻醉颉颃剂的催醒机制。将18只大鼠随机分为C组(对照组)、J组(麻醉颉颃剂组)。J组又分为2个亚组,即J1组(注射小型猪专用复合麻醉颉颃剂5min)、J2组(注射小型猪专用复合麻醉颉颃剂1h)。各组大鼠到达相应的时间点后断头处死,分离大脑皮层、小脑、海马、脑干和丘脑,用Western blot的方法检测p-p38蛋白的表达量,实时荧光定量PCR方法检测c-myc基因mRNA的转录量。试验结果显示大鼠大脑皮层、丘脑和脑干的p-p38蛋白和c-myc mRNA的相对表达量显著升高,而小脑和海马的p-p38蛋白和c-myc mRNA的相对表达量显著降低。综合试验结果,小型猪复合麻醉颉颃剂能够影响p-p38蛋白和c-myc mRNA的表达,这可能是其产生催醒作用的机制之一。In order to investigate the effects of antagonist for the Combined Anaesthetic for Miniature Pigs(XFM)on p-p38 protein and c-myc gene in different encephalic region of rats,and discuss the mechanism of XFM antagonist.Eighteen Wistar rats were divided randomly into C(Control group)and J(Antagonist group)group.J group consisted of two subgroups,J1(5min after rats received the XFM antagonist intraperitoneally)and J2(1hafter rats received the XFM antagonist intraperitoneally).The brain tissues were collected at each time point.The expression of p-p38 in different brains regions were detected by Western blot.The transcription of c-myc mRNA in different brains regions were detected by real time PCR.The results showed that the expression of p-p38 and c-myc mRNA in cerebral cortex,thalamus and brain stem increased significantly;the expression of p-p38 and c-myc mRNA in cerebellum and hippocampus decreased significantly.These results indicated that p-p38 protein and c-myc gene were affected by the XFM antagonist,this may be one of the antagonism mechanisms.

关 键 词：小型猪专用复合麻醉颉颃剂 催醒机制 p-p38蛋白 C-MYC mRNA 

分 类 号：S859.791[农业科学—临床兽医学]