维生素E对小鼠体内精子品质及精子DNA的保护作用  

The Protection of Vitamin E on Quality and Integrity of Mice Semen invivo

作　　者：赵宪林[1] 曹少杰[2] 赵春平[1] 郝瑞杰[1] 桂林生[1] 昝林森[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]渭南职业技术学院渭南市农产品食品检验检测研究中心,渭南714000

出　　处：《畜牧兽医学报》2015年第2期332-338,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十二五”国家863计划(2011AA100307-02);国家自然科学基金(31272411);新疆建设兵团重大科技计划(2014AA001-1);“十二五”国家转基因育种重大专项(2014ZX08007-002)

摘　　要：旨在研究维生素E对小鼠精子DNA的保护作用及对精液中抗氧化物酶活性的影响。以小鼠为动物模型,选日龄、大小相同的健康雄性小白鼠50只,随机分成5组,在试验组小鼠的每千克日粮中分别添加4、8、12、16g的维生素E,对照组添加量为0,饲喂30d后,通过颈椎脱臼处死小鼠,解剖后通过附睾和曲精细管获得小鼠精液。通过单细胞凝胶电泳及相关检测试剂盒检测分析各组间精液DNA的完整性、精子活力、密度、畸形率、精浆中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)、谷胱甘肽还原酶(GR)和过氧化氢酶(CAT)的活性。结果表明:每千克日粮中维生素E的添加量为12g时,0级精子百分率与对照组相比差异显著(P<0.05);添加量为8~16g时,精子活力与对照组相比差异显著(P<0.05);GR和SOD酶的活性与对照组相比差异显著(P<0.05)。每千克日粮中维生素E添加量为12~16g时,CAT酶的活性与对照组相比差异显著(P<0.05)。综上表明,在饲粮中添加维生素E浓度为12g·kg-1时可以明显提高小鼠精液中CAT、GR、GSH和SOD酶的活性及精子DNA的完整性。This study aimed to investigate the effects of vitamin E added in diet on sperm DNA integrity and antioxidant activities of semen in mice.50 mice divided into 5groups were fed in the experiment,in which vitamin E was added in diet with the different content of 0(Control),4,8,12 and 16g·kg-1,respectively.The mice were killed by cervical vertebra dislocation and dissected after 30 days when semen was obtained from epididymis and seminiferous tubules.The sperm motility,density,deformity rate and DNA integrity were detected by single cell gel electrophoresis(SCGE)assay and test kits,as well as the antioxidant activities including superoxide dismutase(SOD),glutathione peroxidase(GSH),glutathione reductase(GR)and catalase(CAT).The percentage of'grade 0'sperm in diet supplemented with 12g·kg-1 vitamin E was significantly higher than that of other treatment groups(P<0.05).The sperm motility was significantly im-proved(P<0.05)and the levels of GR and SOD in seminal plasma were significantly higher(P<0.05)by the supplementing with 8-16g·kg-1 vitamin E in diet than the control,respectively.The CAT activity in seminal plasma reached the highest level with supplementation of 12-16g·kg-1 vitamin E in diet,when compared to the others(P<0.05).In conclusion,we could infer that 12g·kg-1 vitamin E added could obviously improve the sperm DNA integrity and the antioxidant activities of CAT,GR,GSH and SOD in seminal plasma.

关 键 词：小鼠 维生素E 精子 DNA完整性 

分 类 号：R698.2[医药卫生—泌尿科学]