小反刍兽疫病毒化学合成表位多肽对小鼠的免疫效果研究  

Research of the Immune Efficacy of Chemical Synthetic Epitope Polypeptide of Peste des Petits Ruminants Virus on Mice

作　　者：刘文华[1] 王志亮[2] 吴晓东[2] 孙成友[2] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]中国动物卫生与流行病学中心国家外来动物疫病诊断中心,青岛266032

出　　处：《畜牧兽医学报》2015年第3期438-444,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：利用化学合成的小反刍兽疫病毒(PPRV)表位多肽免疫小鼠,以期了解表位多肽的免疫效果。首先利用表位预测软件筛选针对PPRV F和H基因的4条表位多肽,并进行化学合成。将合成多肽免疫小鼠,通过ELISA、MTT法、NK细胞杀伤活性测定及T细胞亚群检测等免疫试验分析预测表位的抗原性。结果表明:在筛选的4条表位多肽中,H242-255、F528-541和H349-362具有较好的体液免疫功能;H242-255、H392-405具有较好的细胞免疫功能。其中H242-255多肽能够满足两方面的功能,有望作为候选表位疫苗成分。试验结果为筛选有效表位用于PPRV表位疫苗的研究奠定了基础。Epitope polypeptides of peste des petits ruminants virus(PPRV)were synthesized and its immunogenicity was examined in mice.Four epitope polypeptides against F and H gene of PPRV were selected by the epitope prediction software and acquired by chemical synthesis,then were administered subcutaneously at dose of 100μg to BALB/c mice,and the epitope antigenicity was analyzed by immunoassays of ELISA,MTT,the killing activity of NK cell and T lymphocyte subsets detection.As a result,H242-255,F528-541 and H349-362 had better humoral immune function among the four epitope polypeptides,while H242-255 and H392-405 had better cellular immune function.Thus,peptide of H242-255 can satisfy the two immunologic functions,and can be used as a candidate epitope vaccine component.The study had great significance by screening efficient epitopes for the development of PPRV epitope vaccines.

关 键 词：小反刍兽疫病毒 表位多肽 NK细胞杀伤活性 MTT法 T细胞亚群检测 

分 类 号：S852.65[农业科学—基础兽医学]