实验感染肝片吸虫和田羊和中国美利奴羊绵羊免疫反应  

Study on Immune Response in Hetian Sheep and Chinese Merino Sheep Infection with Fasciola hepatica

作　　者：邢凤[1,2] 梁志朋[1,2] 李成林[1,2] 

机构地区：[1]塔里木大学动物科学学院,阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,阿拉尔843300

出　　处：《畜牧兽医学报》2015年第3期445-452,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：塔里木大学校长基金博士项目(TD2KBS201203)

摘　　要：以和田羊和中国美利奴羊为研究对象,随机分为感染组(n=5)和对照组(n=3),感染组每只羊经口感染250个囊蚴,在感染后的0、3、6、10周测定血液中生化指标、细胞因子(IFN-γ、IL-2、IL-10、TNF-α)及免疫球蛋白(IgG、IgM、IgA)含量。感染6周后,和田羊和中国美利奴羊感染组血清谷草转氨酶(AST)、总胆红素(TB)及碱性磷酸酶(ALP)含量均显著上升,显著高于对照组(P<0.05)。在感染6、10周后,中国美利奴羊感染组血清球蛋白(GLB)含量显著高于对照组(P<0.05)。和田羊血清IgM含量在感染6周后感染组显著高于对照组(P<0.05);在感染6、10周后,这两个品种的绵羊感染组血清IgG、IL-2、IFN-γ的含量均显著上升,显著高于对照组(P<0.05)。在感染的过程中,中国美利奴羊血清谷草转氨酶(AST)、血清碱性磷酸酶(ALP)含量比和田羊上升幅度大,而和田羊血清IgM、IgG、TNF-α及IFN-γ含量比中国美利奴羊上升幅度大。结果提示,和田羊和中国美利奴羊对肝片吸虫的免疫反应存在一定的差异,本研究为绵羊抗肝片吸虫病的研究提供了理论资料。In this study,Hetian sheep and Chinese Merino sheep were chosen as the research object.They were randomly divided into infection group(n=5)and control group(n=3).Every sheep in infection group was orally infected with 250 metacercariaes of Fasciola hepatica,the biochemical index,cytokines(IFN-γ,IL-2,IL-10,TNF-α)and immunoglobulin(IgG,IgM and IgA)in blood were measured at 0,3,6,10 weeks after infection.In Hetian sheep and Chinese Merino sheep,the content of the aspertate aminotransferase(AST),total bilirubin(TB)and alkaline phosphatase(ALP)in serum of the infection group were significantly increased,significantly higher than that of the control group at 6weeks after infection(P<0.05),the serum globulin(GLB)content of the infection group in Chinese merino sheep were significantly higher than that of the control group at 6and 10 weeks after infection(P<0.05).The serum IgM content of the infection group in Hetian sheep were significantly higher than that of control group at 6weeks after infection(P<0.05).At 6and 10 weeks after infection,the level of IgG,IL-2and IFN-γwere significantly increased,significantly higher than of the control group(P<0.05).The level of ser-um AST,ALP in Chinese Merino sheep increased more than that of Hetian Sheep,however,the levels of serum IgM,IgG,TNF-αand IFN-γin Chinese Merino sheep increased more than that of Hetian Sheep.The results suggest that there are some differences on immune response against Fasciola hepatica between Hetian sheep and Chinese Merino sheep,the study provides some theoretical data for sheep anti-Fascioladisease research.

关 键 词：绵羊 肝片吸虫 免疫球蛋白 细胞因子 免疫反应 

分 类 号：S858.26[农业科学—临床兽医学]