山东地区鸡传染性支气管炎病毒抗原相关性和血清分型  

Antigenic Relatedness and Serotype of Infectious Bronchitis Virus Isolated in Shandong Province

作　　者：胡北侠[1] 许传田[1] 杨少华[1] 黄艳艳[1] 张琳[1] 黄庆华[1] 霍亚飞 张伟[1] 张秀美[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室,济南250100 [2]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2015年第4期615-623,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：现代农业产业技术体系建设专项资金(CARS-42-Z12)

摘　　要：为进一步明确山东地区鸡传染性支气管炎病毒(IBV)的流行病学,对IBV分离株之间以及与疫苗株之间的抗原相关性进行了分析。分别制备了3个疫苗株(4/91、H120和Ma5)和18个分离株的单因子阳性血清,通过鸡胚交叉中和试验测定了阳性血清对同源病毒和异源病毒的中和效价,计算了不同毒株之间的抗原相关系数(R值),并根据R值进行血清分型。结果显示,3个疫苗株和18个分离株可分为5个血清型。疫苗株4/91属于血清Ⅰ型,H120、Ma5与2个分离株(SDWF0608和SDLY0701)属于同一血清型,分离株SDZB0808与SDLY0702、CK/CH/SD08/007和CK/CH/SD09/001等15个毒株为同一血清型,CK/CH/SD09/006和CK/CH/SD10/001分别与其他3和6个分离株组成两个血清型。进一步分析发现,部分IBV毒株之间表现单向中和反应性,属于同一血清型的IBV毒株之间抗原相关性可能存在明显差异。本研究结果一方面表明IBV毒株之间复杂的抗原相关性,另一方面显示IBV流行株与3个疫苗株之间抗原性存在明显差异。To clarify the epidemiology of infectious bronchitis virus(IBV)in Shandong province,the antigenic difference among field isolates and vaccine strains was studied.The monovalent antisera against 3commercial IBV vaccine strains(H120,Ma5 and 4/91)and 18 field isolates were prepared in SPF chickens.The cross viral neutralization(VN)test was performed in SPF chicken embryos to determine the endpoint titers for homologous and heterologous neutralization.The serotype of IBV strains was determined based on antigenic relatedness values(ARV)calculated by the formula R= r槡1×r2.As a result,vaccine strain 4/91 was belong to serotype I.Vaccine strain H120,Ma5 and two isolates(SDWF0608and SDLY0701)were grouped in the same serotype.SDZB0808 and the other 15 isolates were grouped in the same serotype.CK/CH/SD09/006 and CK/CH/SD10/001 were in 2other different serotypes,together with other 3or 6IBV isolates,re-spectively.Moreover,some IBV strains showed one-way neutralizing reaction with others and some IBV strains in the same serotype had different ARVs.The resultes indicate that the antigenicity of IBV strains is very complicated and there are evident antigenic difference between IBV isolates and 3vaccine strains.

关 键 词：鸡传染性支气管炎病毒 交叉中和试验 抗原相关性 血清型 

分 类 号：S852.65[农业科学—基础兽医学]