感染新型鸭呼肠孤病毒雏番鸭和健康雏番鸭的肝蛋白质组学的差异  

Differential Proteomics in Livers of Muscovy Ducklings in Health and Challenged with Novel Duck Reovirus

作　　者：朱果真[1,2] 黄梅清[1,2] 陈仕龙[1,2] 郑敏[1,2] 程晓霞[1,2] 陈少莺[1,2] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013 [2]福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《畜牧兽医学报》2015年第5期808-814,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31172334);国家863项目(2011AA10A209);福建省自然科学基金(2014J01106)

摘　　要：应用差异蛋白质组学研究感染新型鸭呼肠孤病毒(NDRV)番鸭后的肝和正常番鸭肝的蛋白质组差异,为深入研究NDRV的致病机制奠定良好理论基础。通过二维双向凝胶电泳技术和MALDI-TOF-TOF质谱仪分析获取NDRV感染组与非感染对照组之间的差异表达蛋白质,并运用蛋白质搜索鉴定软件Mascot 2.3.02鉴定蛋白质。结果共鉴定出26个差异表达蛋白质。感染组和非感染对照组比较,感染组肝中有9个表达下调的蛋白质和12个表达上调的蛋白质,3个表达蛋白质仅出现在非感染组,2个表达蛋白质仅出现在感染组。NDRV感染组的番鸭肝有肝细胞受损现象。差异蛋白质组学为NDRV分子发病机制的分析提供了一个新途径。The purpose of this paper was to study the pathogenesis of novel duck reovirus(NDRV)infected muscovy duckling.Forty-four healthy muscovy ducklings were divided into treatment group challenged with NDRV and control one injected with Hank′s.Livers were collected from both groups at the 5th day after challenge.Proteomics of the livers were determined by differential proteomic technology.Differential protein spots between both groups were performed and analyzed by two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometer,and then they were identified by protein search software,Mascot 2.3.02.Twenty-six differential protein spots were separated and identified.Nine down-regulated protein spots and twelve up-regulated protein spots were observed in the liver samples of the treatment after comparison between the two groups.Three protein spots were appeared only in the livers of control and two protein spots are only emerged in the livers of treatment.The results indicate that differential proteomics in livers,which were impacted and damaged by NDRV may provide a new idea of pathogenesis of NDRV infected muscovy duckling.

关 键 词：番鸭 肝 差异蛋白质组学 新型鸭呼肠孤病毒 

分 类 号：S858.32[农业科学—临床兽医学]