老龄牦牛睾丸组织结构研究  

The Histologic Characters of Testis in Aging Yak

作　　者：袁莉刚[1] 朱峻峰[1] 谷来凤[1] 闫振龙[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《畜牧兽医学报》2015年第5期855-862,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31160488);甘肃省自然科学研究基金(1010RJZA210);甘肃省教育厅科技项目(0902B-03)

摘　　要：探讨高原老龄牦牛睾丸组织结构特点与其生殖机能的关系。采集9~13岁健康老龄牦牛睾丸组织,应用组织化学染色和透射电镜技术,观察其细胞化学特点及结构特征。结果显示,老龄牦牛睾丸体积及重量左侧略大于右侧,光镜下睾丸被膜、间质组织胶原纤维及网状纤维丰富,小叶不明显,Leydig细胞散在于结缔组织之间,生精小管不同程度退化,生精上皮层数为2~5层,细胞间隙变大,部分脱落,Sertoli细胞低矮,细胞质较少。电镜观察Sertoli细胞核外形不规则,内质网呈疏松的泡状,次级溶酶体增加,体积增大。相邻Sertoli细胞间没有观察到并行的内质网层和细丝束这一血-睾屏障所特有外质特化区形态结构,部分生精小管固有膜胶原纤维增生,上皮皱缩;Leydig细胞内脂滴异常丰富,毛细血管内皮细胞间存在紧密连接。间质组织偶有肥大细胞,间质血管及生精小管固有膜中PAS及AB-PAS阳性反应明显。老龄牦牛Sertoli细胞细胞器结构异常,血-睾屏障中Sertoli细胞间的连接处缺陷,且Sertoli细胞与基膜连接的改变可能影响了Sertoli细胞和生精细胞之间的相互作用,进而抑制了生精细胞的功能。This study was conducted to characterize the histologic structure of aging yak testis.The testis of aging yak in age from 9to 13 years old were prepared for light and electron microscopy by histochemistry and transmission electron methods.The volume and weight of aging yak left testis were slightly larger than the right,and the observations made with the light microscope were obvious morphological changes of aging,the Leydig cells were scattered in the interstitial connective tissue which were abundant with collagen and reticular fiber between the seminiferous tubules,there were partly progressive regressed in the seminiferous epithelium and the epithelium were constructed 2-5layers with contained large intercellular,the spermatogenic cells partially desquamated and Sertoli cells were low with less cytoplasm.The electron microscopic studies show that the Sertoli cells were irregularly shaped nuclei and the endoplasmic reticulum were represented as a loose,vesiculated network,and the lysosomes were large in shape and few in number.The typical morphological configuration of the blood-testis barrier as microfilaments constructed by the typical ectoplasmic specialization and actin filament between the adjacent Sertoli cells were not seen in aged yak.Partial lamina propria of seminiferous epithelium was shrinkage and hyperplasia of collagen fiber.The lipid droplets in Leydig cells were very rich,occasionally,the mast cell can be seen in the interstitial tissue.Moreover,there are closely connection between the adjacent interstitial capillary endothelial cells and the positive reaction of PAS and AB-PASwere obviously present throughout the basement membranes and the wall of interstitial blood vessels.Taken together,the structural abnormalities in Sertoli cells,the connection defects in bloodtestis barrier and the basement membrane may affect the interaction between Sertoli cells and spermatogenic cells,thereby the functions of aging yak testis were inhibited.

关 键 词：牦牛 老龄 睾丸 组织化学 超微结构 

分 类 号：S823[农业科学—畜牧学]