新疆阿拉山口口岸地区鼠类棘球蚴感染情况调查  

Investigation on Echinococcosis in Wild Rodents at Ala-shankou Port

作　　者：肖云霞[1] 徐新龙[2] 尹小平[2] 王安东[1] 闫遵祥 段成任 徐军[2] 

机构地区：[1]石河子大学动物科技学院,石河子832000 [2]阿拉山口出入境检验检疫局,阿拉山口833418 [3]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《畜牧兽医学报》2015年第6期1004-1010,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家质检总局科技计划项目(2014IK239)

摘　　要：对新疆阿拉山口口岸地区鼠类棘球蚴感染情况进行系统调查。选择阿拉山口口岸三个不同区域(野外区、城郊综合区和城区),2013—2014年连续2年采集鼠类样本;剖检、分离肝,对所有样本提取肝基因组DNA,利用聚合酶链式反应扩增棘球蚴线粒体细胞色素C氧化酶亚单位1(Cox1)、NADH脱氢酶(ND1)等基因并测序。剖检发现8份样品具有包囊,感染率为2.08%;分子鉴定发现有10份样品呈PCR阳性,感染率为2.60%,Cox1基因Blast分析中有8份样品与青海检测的苏俄多房棘球绦虫的相似性最高(登录号:JQ 690286.1),为85%;ND1基因Blast分析,另2份样品与多房棘球蚴相似性最高(登录号:AB 720065.1),为100%;统计学分析发现野外区、城郊综合区鼠类感染率分别为2.74%和2.38%,而城区未检测到病原体。阿拉山口口岸野外区野鼠棘球蚴感染率最高,易感鼠种为大沙鼠。The infectious status of echinococcosis in wild rodents was investigated at different areas of Alashankou Port.The wild rodent samples were collected from rural,suburb and urban area of Ala-shankou Port during 2013-2014.Then,pathological sections of liver were prepared and stained with H&E;the genomic DNA of livers and cysts were extracted.Cytochrome C oxidase 1(Cox1),NADH dehydrogenase subunit 1(ND1)in Echinococcus mitochondria were amplified from the DNA and sequenced.Results were as follows:eight samples with cyst were found in the process of autopsy,and the positive rate was 2.08%.Ten positive samples were detected positive by PCR,and the positive rate was 2.60%.The analysis of Blast showed that 8samples of our sequences were clustered with that of Echinococcus sp.(Accession No.JQ 690286.1),and their identities were 85%.The remaining 2samples of our sequences were clustered with that of Echinococcus multilocularis(Accession NO:AB 720065.1),and their identities were 100%.The results of statitical analysis conveyed us the infection rates of echinococcosis at rural and suburb area were 2.74% and 2.38%,respectively,while no positive sample was detected at urban area.The results presented here demonstrate that the wild rodents at rural area of Ala-shankou Port showed the highest infection rate,and its susceptible host was the Rhombomys opimus.

关 键 词：棘球蚴病 野鼠 阿拉山口口岸 

分 类 号：S855.9[农业科学—临床兽医学]