沙葱黄酮对绵羊外周血淋巴细胞增殖及IL-2、IL-4、IFN-γ mRNA表达的影响  

Effect of Allium Mongolicum Regel Flavonoids on Lymphocyte Proliferation,mRNA Expression of IL-2,IL-4,IFN-γ in Peripheral Blood Lymphocyte of Sheep

作　　者：萨茹丽[1] 木其尔[1] 王翠芳[1] 包玲玲[1] 敖长金[1] 王思珍[2] 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018 [2]内蒙古民族大学动物科学与技术学院,通辽028000

出　　处：《畜牧兽医学报》2015年第6期1063-1070,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金地区科学基金项目(31260558;31160474);"十二五"国家科技支撑计划(2013BAD10B04)

摘　　要：本试验以绵羊外周血淋巴细胞为研究对象,研究沙葱黄酮对绵羊外周血淋巴细胞增殖率,IL-2、IL-4、IFN-γ基因mRNA表达量的影响,为全面揭示沙葱黄酮的免疫调节活性机制和相关产品的开发提供试验依据。试验采用MTT法研究沙葱黄酮对绵羊外周血淋巴细胞增殖率的影响,使用RT-PCR法研究沙葱黄酮对绵羊外周血淋巴细胞IL-2、IL-4、IFN-γ基因mRNA表达量的影响。结果显示,沙葱黄酮具有促进绵羊外周血淋巴细胞增殖的作用,对绵羊外周血淋巴细胞IL-2mRNA表达无明显调节作用,可上调IFN-γmRNA表达,下调IL-4mRNA表达。综上,沙葱黄酮具有促进绵羊外周血淋巴细胞增殖及免疫调节活性的作用。In order to study the action mechanism of Allium Mongolicum Regel Flavonoids(AMF)on immunological enhancement activity,the effects of AMF on lymphocyte proliferation and mRNA expression of IL-2,IL-4and IFN-γ were determined by MTT method and real-time fluorescent quantitation PCR assay.The peripheral blood lymphocyte of sheep was used in this study.The results showed that AMF could significantly enhance lymphocyte proliferation.AMF could significantly up-regulate mRNA expression of IFN-γ,down-regulate mRNA expression of IL-4,and have no significantly effect on IL-2 mRNA expression.The result indicate that AMF has immunoregulatory activity and could enhance lymphocyte proliferation.

关 键 词：沙葱黄酮 淋巴细胞增殖 IL-2 IL-4 IFN-Γ 

分 类 号：S853.7[农业科学—临床兽医学]