代谢综合征患者血浆甘油三酯水平对HDL亚类分布的影响  

Effect of Plasma Triglyceride Concentration on the Distribution of HDL Subclasses in Patients with Metabolic Syndrome

作　　者：李娜[1] 龙石银[1] 陈志军[1] 陈浩[1] 陈武哲[1] 肖梨花 欧阳超[1] 田英[1] 乔新惠[1] 

机构地区：[1]南华大学生物技术系,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2015年第3期285-289,共5页Chinese Journal of Arteriosclerosis

基　　金：湖南省科技厅基金项目(2013SK3120);湖南省教育厅项目(12C1246);湖南省高层次卫生人才"225"工程项目基金;衡阳市科技局项目(2013KJ05);南华大学研究生科研创新项目(2013XCX20);南华大学"十二五"科技创新团队基金;南华大学留学归国人员启动基金;南华大学大学生创新项目

摘　　要：目的探讨代谢综合征(MS)患者血浆甘油三酯(TG)水平对高密度脂蛋白(HDL)亚类分布的影响。方法选取在南华大学附属第一医院就诊的MS患者血样,全自动生化分析仪测定血脂含量及载脂蛋白浓度,根据美国国家胆固醇教育计划NCEP ATP-Ⅲ文件,将MS患者按TG浓度分4组,即TG<1.69 mmol/L、1.69mmol/L≤TG<2.25 mmol/L、2.25 mmol/L≤TG<5.64 mmol/L、TG≥5.64 mmol/L,采用双向电泳-免疫印迹检测法测定MS患者和99例正常人血浆HDL亚类的相对含量。结果与对照组相比,MS患者血浆总胆固醇(TC)、TG、低密度脂蛋白胆固醇(LDLC)、载脂蛋白B100(Apo B100)、preβ1-HDL、HDL3b含量及Apo B100/AⅠ和LDLC/HDLC比值均显著性增高(P<0.05或P<0.001),HDLC、Apo AⅠ、HDL2b、HDL2a显著降低(P<0.05或P<0.001)。并且随着血浆TG水平的升高,小颗粒的preβ1-HDL、HDL3a和HDL3b含量升高,而大颗粒的HDL2b和HDL2a含量降低。结论MS患者HDL亚类分布异常,小颗粒的preβ1-HDL和HDL3b含量升高,而大颗粒的HDL2b和HDL2a含量降低,血浆TG含量变化可能是影响MS患者HDL亚类异常的因素之一。Aim To investigate the association between high density lipoprotein( HDL) subclasses and plasma triglyceride( TG) concentration in metabolic syndrome( MS). Methods Concentrations of plasma lipids and the apolipoproteins were detected by automated biochemical analyzer. According to the NCEP ATP-Ⅲ file set by the U. S. National Cholesterol Education Program,the MS subjects were divided into four groups of TG < 1. 69 mmol / L,1. 69 mmol / L≤TG <2. 25 mmol / L,2. 25 mmol / L≤TG < 5. 64 mmol / L,TG≥5. 64 mmol / L. HDL subclasses were determined by two-dimensional gel electrophoresis associated with immunodetection. Results Compared with their counterpart control,the levels of plasma total cholesterol( TC),TG,low density lipoprotein cholesterol( LDLC),apolipoprotein B100( Apo B100),preβ1-HDL,HDL3 b,Apo B100/ AⅠ and LDLC / HDLC were significantly increased in MS( P < 0. 05 or P < 0. 001). Meanwhile,the concentrations of HDLC,Apo AⅠ,HDL2 band HDL2awere significantly decreased in MS( P < 0. 05 or P < 0. 001). The content of preβ1-HDL,HDL3 aand HDL3bwere increased,while the HDL2 aand HDL2bwere significantly decreased,with the increasing of plasma TG levels in MS. Conclusion HDL subclasses distribute abnormal in MS,the content of preβ1-HDL and HDL3 bwere increased,while HDL2 band HDL2awere significantly decreased in MS. The levels of plasma TG may be one of the influencing factors of abnormal HDL subclasses in patients with MS.

关 键 词：代谢综合征 HDL亚类 甘油三酯 双向电泳-免疫印迹法 

分 类 号：R589.2[医药卫生—内分泌]