高脂蛋白(a)的冠心病患者内皮祖细胞功能受损  

Impaired Function of Endothelial Progenitor Cells in High Concentration Lipoprotein(a) Coronary Artery Disease Patients

作　　者：白洁[1] 孟军[1] 蔡泽民[1] 何谨[1] 童海[1] 王佐[2] 

机构地区：[1]南华大学附属第一医院,湖南省衡阳市421001 [2]南华大学心血管疾病研究所动脉硬化学湖南省重点实验室,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2015年第4期384-388,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助(81070221)

摘　　要：目的比较高脂蛋白(a)[Lp(a)]的冠心病患者和低Lp(a)的冠心病患者内皮祖细胞(EPC)的功能差异。方法酶联免疫法测定Lp(a),差速贴壁法分离EPC,Dil-ac-LDL吞噬及lectin结合鉴定EPC并计数。MTT法检测EPC存活与增殖,改良Boyden小室检测细胞迁移,明胶玻片法检测细胞黏附,杂交瘤皿上观察并计数单个细胞克隆数,基质胶上测量管状结构形成长度。结果高Lp(a)的冠心病患者循环EPC数显著低于低Lp(a)的冠心病患者(109.4±13.8个/视野比384.0±37.0个/视野,P=0.0023);MTT分析显示,低Lp(a)组OD值为0.77±0.05,而高Lp(a)组OD值为0.23±0.04(P=0.0018),表明高Lp(a)的冠心病患者EPC的生存状态较差,这一点在凋亡检测中进一步得到体现,高Lp(a)组EPC凋亡率显著高于低Lp(a)组(14.9%±3.3%比4.1%±0.8%,P=0.035)。与低Lp(a)组比较,高Lp(a)组EPC的黏附(25.3±4.6个/视野比78.6±6.8个/视野,P=0.0030)、迁移(22.0±2.6个/视野比56.0±4.9个/视野,P=0.0037)、克隆形成(2.4±0.4个/视野比11.0±1.3个/视野,P=0.0003)、管状结构形成(7.4±1.2 mm/field比33.3±2.6 mm/field,P=0.0001)均显著受损。结论与低Lp(a)的冠心病患者相比,高Lp(a)的冠心病患者EPC功能受损更严重。Aim To compare the functional difference of endothelial progenitor cells(EPC) of high concentration Lp( a)( ≥300 mg / L) coronary artery disease( HLPCAD) patients and low concentration Lp( a)( < 300 mg / L) coronary artery disease( LLPCAD) patients. Methods Differential adherence method was used to isolate EPC,Dil-acLDL swallowed and lectin binding was used for EPC identification. MTT was used to assay EPC survival and proliferation,modified Boyden chamber for migration,gelatin slide method for adhesion,a single cell hybridoma clones dish was observed and counted,and tubular structures formed on matrigel matrix length was measured. Results The numbers of circulating EPC in HLPCAD patients were significantly lower than those in LLPCAD patients( 109. 4 ± 13. 8 Cells / field vs. 384. 0 ± 37. 0 Cells / field,P = 0. 0023). MTT analysis showed that the OD value of LLPCAD group was 0. 77 ± 0. 05,and the HLPCAD was 0. 23 ± 0. 04( P = 0. 0018),the apoptosis rate of HLPCAD EPC was significantly higher than that in LLPCAD group(14. 9% ± 3. 3% vs. 4. 1% ± 0. 8%,P = 0. 035). The numbers of adhesion(25. 3 ± 4. 6 Cells / field vs.78. 6 ± 6. 8 Cells / field,P = 0. 0030),migration(22. 0 ± 2. 6 Cells / field vs. 56. 0 ± 4. 9 Cells / field,P = 0. 0037),and clone-form units(2. 4 ± 0. 4 number / field vs. 11. 0 ± 1. 3 number / field,P = 0. 0003),tubular structure formation(7. 4 ±1. 2 mm / field vs. 33. 3 ± 2. 6 mm / field,P = 0. 0001) of HLPCAD patients were significantly reduced. Conclusions Function of HLPCAD EPC is seriously impaired while compared with LLPCAD EPC.内皮祖细胞(endothelial progenitor cell,EPC)是

关 键 词：脂蛋白(a) 内皮祖细胞 细胞迁移 细胞增殖 管状结构形成 

分 类 号：R541.4[医药卫生—心血管疾病]