白头翁皂抑制佐剂性关节炎大鼠成纤维样滑膜细胞凋亡  

Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats

作　　者：缪成贵[1,2,3] 周国梁[1] 秦梅颂[1] 陈建中[1] 李成凤[1] 何华奇[4] 

机构地区：[1]安徽科技学院食品药品学院药物制剂教研室,安徽凤阳233100 [2]安徽医科大学药学院基础与临床药理学教研室,合肥230032 [3]安徽科技学院家禽疫病监测安徽省重点实验室,安徽凤阳233100 [4]安徽科技学院生命科学学院园艺教研室,安徽凤阳233100

出　　处：《中南大学学报（医学版）》2015年第2期144-149,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81302783);安徽科技学院科研项目(ZRC2014473);滁州市科技局科研项目(201306);安徽省优秀青年人才项目(98)~~

摘　　要：目的:研究白头翁皂苷(pulchinenoside,PULC)对佐剂性关节炎(adjuvant arthritis,AA)大鼠成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)凋亡的影响。方法:60只SD大鼠被随机分为正常对照组、AA组、PULC低剂量组(50 mg/kg)、PULC中剂量组(100 mg/kg)、PULC高剂量组(150 mg/kg)、布洛芬8 mg/kg阳性药物组,每组10只。原代培养AA大鼠FLS,real time q PCR检测PULC灌胃治疗对AA大鼠FLS抗凋亡基因Bcl-2,促凋亡基因Bax和细胞凋亡因子caspase-3表达的调控作用;MTT检测PULC治疗对FLS增殖的影响;ELISA检测PULC对FLS的IL-6和IL-8表达的影响。结果:与AA组比较,各治疗组FLS中抗凋亡基因Bcl-2表达明显降低(均P<0.05),促凋亡基因Bax和细胞凋亡因子caspase-3表达显著升高(均P<0.05),AA大鼠FLS增殖活力明显降低(均P<0.05),IL-6和IL-8表达显著下降(均P<0.05)。结论:PULC可能通过促进FLS凋亡而抑制AA大鼠滑膜的异常增殖。Objective: To explore the eff ect of pulchinenoside(PULC) on fibroblast-like synoviocytes(FLS) apoptosis in adjuvant arthritis(AA) rats.Methods: A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group(50 mg/kg), a middle PULC group(100 mg/kg) or a high PULC group(150 mg/kg) and an ibuprofen(8 mg/kg) group(n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qP CR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.Results: Compared with the AA group, the Bcl-2 expression was down-regulated(all P<0.05), the Bax and caspase-3 expression was up-regulated(all P<0.05), and the FLS proliferation was inhibited(all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages(all P<0.05) as well as in the ibuprofen group(P<0.05).Conclusion: PULC may inhibit the FLS proliferation in AA rats by increase in FLS apoptosis.

关 键 词：白头翁皂苷 佐剂性性关节炎 Bcl-2 BAX caspase-3 

分 类 号：R285.5[医药卫生—中药学]