急性心肌梗死患者血清循环miR-214水平及其与冠状动脉病变范围的关系  

Circulating miR-214 level and its correlation with the extent of coronary lesion in patients with acute myocardial infarction

作　　者：汪雁归[1] 黄佳[2] 杨天伦[3] 

机构地区：[1]湖南省人民医院老年病科,长沙410001 [2]中南大学湘雅二医院ICU,长沙410011 [3]中南大学湘雅医院心内科,长沙410008

出　　处：《中南大学学报（医学版）》2015年第4期362-366,共5页Journal of Central South University ：Medical Science

基　　金：湖南省科技计划项目(2012SK3243)~~

摘　　要：目的:了解急性心肌梗死(acute myocardial infarction,AMI)患者血清中循环mi R-214表达水平及其与冠状动脉病变范围的关系。方法:收集2011年9月至2012年1月在中南大学湘雅医院心内科住院的AMI患者45例(AMI组),并选择同时期在中南大学湘雅医院体检中心体检的20例健康人员为对照组。AMI组按冠状动脉病变的范围又分为单支、双支和三支病变组(n=20,13,12)。提取各组人员入院时血清中总RNA,并行荧光定量PCR检测循环mi R-214表达水平,酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测患者空腹血浆中胎盘生长因子(placental growth factor,PLGF)分泌水平。比较不同冠状动脉病变支数组间循环mi R-214和PLGF水平,并分析上述指标与AMI病变范围的相关性。结果:AMI各亚组血清中循环mi R-214水平低于对照组,且病变范围越广下降水平越明显,各组间对比差异均有统计学意义(均P<0.05);AMI各亚组血浆中PLGF表达水平高于对照组,且随病变范围增大表达水平升高越明显,各组间对比差异均有统计学意义(均P<0.05)。AMI组血清中循环mi R-214水平与血浆中PLGF水平呈负相关(r=-0.588,P<0.01)。结论:AMI组循环mi R-214水平显著降低,且它与AMI的病变范围存在相关性。循环mi R-214有可能作为AMI早期诊断和预后的生物学标志物。Objective: To examine the circulating levels of mi R-214 in acute myocardial infarction(AMI) patients and to explore the relationship between the circulating levels of mi R-214 and the degree of coronary lesion.Methods: A total of 45 patients with AMI(AMI group) were enrolled from Xiangya Hospital of Central South University between September, 2011 and January, 2012. Twenty healthy volunteers served as a normal control group(n=20). According to the coronary artery lesion area, AMI group was also divided into a single-branch group, a double-branch group and a triple-branch group(n=20, 13, 12 respectively). Circulating levels of mi R-214 and plasma levels of placental growth factor(PLGF) were measured by real time-PCR assay and enzyme-linked immunosorbent assay respectively on the day when the patients admitted to the hospital. The plasma levels of mi R-214 and PLGF were compared among the various branch groups. The correlation between mi R-214 and PLGF was analyzed in AMI subgroups.Results: The miR-214 levels in the AMI subgroups were lower than that in the control group. The more decrease in miR-214 level, the larger size of coronary lesion. There was significant difference in the different groups(all P<0.05). Compared with the control group, the levels of plasma PLGF were significantly higher in the AMI subgroups. The more increase in PLGF level, the larger size of coronary lesion. There was significant difference in the different groups(all P<0.05). The plasma levels of miR-214 were negatively correlated with that of PLGF in the AMI group(r=-0.588, P<0.01).Conclusion: The circulating level of mi R-214 was significantly decreased in the AMI group, which might be correlated with the extent of the coronary lesion. Circulating mi R-214 may be a promising biomarker for the diagnosis and prognosis of severe AMI.

关 键 词：循环miR-214 胎盘生长因子 急性心肌梗死 相关性 

分 类 号：R542.22[医药卫生—心血管疾病]