环江香猪FSHβ、ESR及ZAR1基因多态性分析  

Polymorphism Analysis on FSHβ、ESR and ZAR1 in Huanjiang Pig

作　　者：李俊[1] 陈美娟[1] 许钟峯 吕冠霖 赵子榆 石德顺[1] 刘庆友[1] 

机构地区：[1]亚热带农业生物资源保护与利用国家重点实验室,广西大学,南宁530004

出　　处：《基因组学与应用生物学》2015年第3期495-499,共5页Genomics and Applied Biology

基　　金：国家高技术研究发展计划(863)项目(2013AA102504);国家自然基金项目(31260552)共同资助

摘　　要：环江香猪是广西著名的地方品种,本研究采用PCR-RFLP、HRM结合测序的方法分析了广西环江香猪繁殖状相关基因FSHβ、ESR和ZAR1基因的多态性。结果显示,首次成功克隆测序了环江香猪ZAR1基因的外显子3,与Gen Bank中的ZAR1基因(DQ231443,gi:83727928)外显子3序列100%同源。针对134头环江香猪检测样本中均未发现FSHβ和ESR基因的多态突变位点。对ZAR1基因的外显子3和部分内含子3测序后发现5个新突变SNP位点,未见有已报道的对猪产仔数产生显著影响的外显子3(C54T)突变。论文显示不同品种猪基因突变多态性亦呈现为不同的模式,研究结果对建立环江香猪基因标记辅助选择方法具有重要意义。Huanjiang pig is a famous local variety in Guangxi. PCR-RFLP and HRM assays were used to genotype three candidate genes which are FSHβ, ESR and ZAR1 for the polymorphism of high fecundity traits in Huanjiang pig. The results showed that the first successful cloning and sequencing of Huanjiang pig ZAR1 gene exon 3 and ZAR1 gene in Gen Bank(DQ231443, gi: 83727928) of exon 3 share the same sequence each other. The polymorphism gene mutations of FSHβ and ESR were not found in 134 test samples. Five SNP sites were found by DNA sequencing in ZAR1 gene Exon3 and part of intron3, but there were no C54 T mutation in exon3 which was reported that have significant impact on litter size. The study shows that the different varieties of pig have different patterns in gene mutation polymorphism, and this finding has important implications for the establishment of genetic marker-assisted selection in Huanjiang pigs.

关 键 词：环江香猪 促卵泡激素β亚基基因 雌激素受体基因 合子阻滞因子1 基因多态性 

分 类 号：S828[农业科学—畜牧学] Q78[农业科学—畜牧兽医]