一种新型聚乙烯亚胺(PEI)载体的制备及表征  

Preparation and Characterization of A Novel Polyethylenimine(PEI)-Based Gene Carrier

作　　者：强娜[1] 梁浩[1] 解芳[1] 陶海霞[1] 李险峰[1] 刘立芳[1] 

机构地区：[1]惠州学院化学工程系,惠州516007

出　　处：《高分子通报》2015年第4期74-80,共7页Polymer Bulletin

基　　金：广东省教育厅项目(2013LYM_0088);惠州市科技计划项目(A512.0226)

摘　　要：设计一种新型的用于传输小干扰核糖核酸(siRNA)聚乙烯亚胺-聚类丙交酯功能聚合物。首先合成一类含功能基团羧基的丙交酯衍生物,对其进行了1 H NMR、GPC、DSC表征,功能基团的密度可以通过与丙交酯共聚的单体的物质的量来调控,利用羧基与聚乙烯亚胺(PEI)分子上的氨基偶合,将小分子量PEI通过共价键连接到可生物降解的高分子主链骨架上,这种方法提供了一条途径来解决分子量与细胞毒性以及转染效率之间相互矛盾的关系。本文进一步研究了此体系在负载siRNA前后粒径及zeta电位的变化,颗粒粒径分布在50~500nm之间,Zeta电位在-9^-35mV之间。A novel polyethylenimine(PEI)-based gene carrier was used for carrying aplasmid encoding small interfering RNA(siRNA).A series of semicrystalline copolymers,especially with functional group carboxyl poly(L-lactide-co-β-malic acid)were synthesized.Random polymers P(LA-co-BMD)were first synthesized through ring-opening copolymerization of L-LA and 3(S)-[(Benzyloxycarbony)methyl]-1,4-dioxane-2,5-dione(BMD)in the presence of dodecanol as initiator and stannous octoate(Sn(Oct)2)as catalyst.The structure of the copolymers was characterized by Nuclear Magnetic Resonance(1H NMR),Gel Permeation Chromatography(GPC),and Differential Scanning Calorimetry(DSC).The density of carboxyl groups were increased with the functional monomer.The PEI was coupling with the copolymer by amino groups.The modification of polymeric vectors has shown higher transfection efficiency and lower cytotoxicity.The micelles showed a significant reduction in surface zeta-potential(-9^-35mV)and a much smaller particle size(50~500nm).

关 键 词：聚乙烯亚胺 小干扰核糖核酸 功能基团 ZETA电位 粒径 

分 类 号：O633.2[理学—高分子化学]