广西野生濒危植物掌叶木遗传多样性的ISSR与SRAP分析  

Genetic Diversity of the Endangered Handeliodendron bodinieri in Guangxi Province by ISSR and SRAP Analysis

作　　者：李雪萍[1] 郭松[2,3] 熊俊飞[1,3] 郭华伟[1] 郑琳[1] 李在留[3] 

机构地区：[1]河南科技大学林学院,河南洛阳471003 [2]南京林业大学江苏省林业生态工程重点实验室,南京210037 [3]广西大学林学院,南宁530004

出　　处：《园艺学报》2015年第2期386-394,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31060053);河南省自然科学基金项目(62580021);广西自然科学基金项目(2011GXNSFB018016)

摘　　要：采用ISSR和SRAP技术对中国野生濒危植物掌叶木[Handeliodendron bodinieri(Lévl.)Rehd.]的5个野生居群的95份材料进行分析,10条ISSR引物共扩增得到114个条带,其中多态性条带68个,多态性条带百分率(PPB)59.65%,掌叶木在物种水平上的Nei’s基因多样性(H)为0.1817,Shannon’s遗传多样性信息指数(I)为0.2792;观测等位基因数(Na)为1.5965,有效等位基因数(Ne)为1.2984,居群间基因间分化度(Gst)为35.17%。15组SRAP引物共扩增出292个条带,其中多态性条带269个,多态性条带百分比为92.12%,物种水平上,掌叶木的Nei’s基因多样性(H)为0.3171,Shannon’s遗传多样性信息指数(I)为0.4758;观测等位基因数(Na)为1.9212,有效等位基因数(Ne)为1.5380,居群间基因间分化度(Gst)为23.17%。结果显示两种标记均能检测到掌叶木具有较高的遗传多样性,掌叶木不同居群间存在一定的遗传分化和基因流动,但遗传分化主要存在于居群内。Genetic diversity of 95 samples of five natural populations of the endangered plant Handeliodendron bodinieri(Lévl.)Rehd. was analyzed by using ISSR and SRAP techniques. A total of 114 bands were obtained with 10 ISSR primers,of which 68 bands were polymorphic with a polymorphic proportion of 59.65%. At the species level,the Nei's genetic diversity(H)was 0.1817,and Shannon's information index(I)was 0.2792. The observed number of alleles was 1.5965 and the effective number of alleles was 1.2984. The differentiation coefficient(Gst)among the populations was 35.17%. Totally,292 bands were obtained with 15 SRAP primer pairs,and 269 bands were polymorphic with a proportion of 92.12%. The Nei's genetic diversity(H)was 0.3171,and the Shannon's information index(I)was 0.4758. The observed number of alleles was 1.9212 and the effective number of alleles was 1.5380. The differentiation coefficient(Gst)among the populations by SRAP analysis was 23.17%. The results showed that both methods could be used to determine the genetic diversities among different populations of H. bodinieri. There was a high genetic diversity in H. bodinieri,and genetic differentiation and gene flow existed between the populations, but the main genetic differentiation occurred largely within the population.

关 键 词：掌叶木 ISSR SRAP 遗传多样性 居群 遗传分化 

分 类 号：S792[农业科学—林木遗传育种]