不同温度预处理对青花菜小孢子胚胎发生的影响  

Effect of Different Pre-treated Temperatures on the Microspore Embryogenesis in Broccoli

作　　者：孙继峰[1] 方智远[1] 袁素霞[1] 李占省[1] 杨丽梅[1] 庄木[1] 张扬勇[1] 孙培田[1] 刘玉梅[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2015年第3期563-568,共6页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-25-A);国家自然科学基金项目(30871708);国家科技支撑计划课题(2013BA D01B04);国家高新技术发展计划(‘863’)项目(2012AA100105);农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：以青花菜(Brassica oleracea L.var.italica Plenck)‘B908’和‘B1092’为材料,通过荧光染色技术对经25、4、32.5和(4→32.5)℃等不同温度预处理的小孢子的分裂方式进行观察,并对出胚率进行统计。结果表明,不同温度预处理的小孢子胚胎发生途径基本相同,均以对称分裂产生胚胎的孢子体发育途径为主(B途径),经历球形胚、心形胚、鱼雷胚,最终形成子叶形胚状体。但是不同温度预处理的小孢子启动胚胎发生的频率不同,(4→32.5)℃预处理后的成胚率最高。B908 and B1092 of broccoli(Brassica oleracea L. var. italica Plenck)were used to be the materials in the research on microspore embryogenesis. The nuclear division patterns and embryo development efficiency of isolated microspores of broccoli pretreated at different temperatures were investigated under a fluorescence microscopy by using DAPI staining. Four pretreated temperatures were 25 ℃,4 ℃,32.5 ℃,or 4 ℃→32.5 ℃(combined treatment). The results showed that the nuclear division patterns were much the same under different temperature pretreatment. The B pathway started from symmetrical division that was the main pathway. The cotyledons-shaped embryo was formed after different developmental stages,microspore divided equally,multicellular structure packaged by the exine,original embryoid,globular embryoid,heart-shaped embryoid,torpedo-shaped embryiod,cotyledonsshaped embryoid. The embryoid development efficiency of isolated microspores of broccoli pretreated at different temperatures was different. The combined treatment(4 ℃→32.5 ℃)induced the highest frequency of microspore derived embryo.

关 键 词：青花菜 游离小孢子培养 发育途径 出胚率 

分 类 号：S635[农业科学—蔬菜学]