黄瓜扩张素蛋白基因CsEXPb1的克隆及表达分析  

Cloning and Expression Analysis of the Expansin Gene CsEXPb1 in Cucumber

作　　者：孟永娇[1] 李季[1] 徐建[1] 张开京[1] 娄群峰[1] 陈劲枫[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2015年第4期679-688,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金重点项目(31430075);国家重点基础研究发展计划(‘973’)项目(2012CB113904);国家‘863’项目(2012AA100202);农业部行业专项(201403032);国家自然基金青年基金项目(31301781);江苏省青年基金项目(BK20130674)

摘　　要：基于黄瓜果实转录组学研究,筛选并克隆了一个扩张素蛋白(β-expansin)基因Cs EXPb1。蛋白序列同源比对发现Cs EXPb1蛋白是一类钙调素类似蛋白(Calmodulin-like Protein,CML),与甜瓜、草莓及番茄扩张素蛋白基因具有较高的相似性;Ex Pasy分析发现Cs EXPb1为亲水性蛋白,亚细胞定位分析证明该蛋白广泛分布在细胞质以及细胞核结构中;q RT-PCR分析发现Cs EXPb1无论是在授粉坐果还是单性结实坐果过程中皆上调表达,而在不授粉败育的果实中下调表达;q RT-PCR也表明Cs EXPb1的表达受到生长素、细胞分裂素和赤霉素等单性结实诱导激素的正向调控,推测Cs EXPb1与黄瓜坐果有关,可能参与了激素诱导的单性结实过程。Based on transcriptome study of cucumber fruit set,a β-expansin gene:Cs EXPb1 was cloned and investigated in present study. Homolog BLAST showed that Cs EXPb1 is belonged to Calmodulin-like Protein family,and has high similarities with the expansin genes of Cucumis melo,Fragaria vesca subsp. vesca and Solanum lycopersicum. The Ex Pasy analysis showed that Cs EXPb1 is a hydrophilic protein;The subcellular localization analysis indicated that Cs EXPb1 localized in plasma membrane,cytoplasm and nucleus;q RT-PCR analysis found that Cs EXPb1 was up-regulate expressed during pollination and parthenocarpy fruit set in cucumber,while down-regulated during fruit abortion;q RT-PCR also showed that the transcription of Cs EXPb1 could be cis-regulated by fruit-set inducing hormones i.e. auxin,cytokinin and gibberellin. Therefore,it was speculated that Cs EXPb1 may be close related to hormone induced parthenocarpic fruit set of cucumber.

关 键 词：黄瓜 扩张素蛋白 CsEXPb1基因 表达分析 坐果 单性结实 

分 类 号：S642.2[农业科学—蔬菜学]