吊兰根负向光性表现与机理研究  

Studies on Performance and Mechanism of Negative Phototropism of Chlorophytum comosum Roots and Its Regulation

作　　者：陈娟[1,2] 孔妤[3] 王忠 程水源[1] 陈鹏 

机构地区：[1]黄冈师范学院生命科学学院经济林木种质改良与资源综合利用湖北省重点实验室大别山特色资源开发湖北省协同创新中心,湖北黄冈438000 [2]扬州大学江苏省作物遗传生理重点实验室生物学博士后流动站,江苏扬州225009 [3]中国科学院上海生命科学研究院,上海200031

出　　处：《园艺学报》2015年第4期706-712,共7页Acta Horticulturae Sinica

基　　金：江苏省博士后基金项目(1402115C);经济林木种质改良与资源综合利用湖北省重点实验室开放基金项目(2013000503);黄冈师范学院2011年度产学研合作项目;江苏省林业三新工程重大项目(lysx201305);国家自然科学基金项目(31270228)

摘　　要：以吊兰气生根经水培后长出的不定根和侧根为试材,研究其在单侧光照射下的负向光性表现及其机理与调控。结果表明:(1)所有吊兰不定根和侧根均具有负向光生长的特性,最大弯曲角度可达88.5°。(2)负向光性发生受蓝紫光诱导而红光无效,接受光刺激的部位为根冠,在一定光强范围内,根弯曲角度随着光强的增加而增大。(3)外源Ca Cl2促进吊兰根负向光性弯曲,而外源La Cl3(钙通道阻断剂)抑制负向光性形成,Ca Cl2可缓解La Cl3的抑制效应。(4)单侧光诱导吊兰根向光侧IAA向背光侧横向运输,而外源Ca2+促进这种单侧光诱导的生长素横向运输,外源La Cl3则抑制这种效应。推测吊兰根负向光性的形成过程大致为根冠细胞受到单侧光刺激后导致IAA从向光侧向背光侧运输,抑制背光侧细胞的生长,从而使两侧细胞生长不均等,而Ca2+作为第二信使介导了这一过程的发生。The aerial roots of Chlorophytum comosum were planted in water to grow hydroponic roots to study the negative phototropism performance and mechanism and its regulation.(1)All the adventitious roots and their branch roots bent away from light,the maximum curvature maybe 88.5°.(2)Photo perception site was root cap. The curvature was increasing when light intensity ranged from 0 to 110 μmol · m-2 · s-1. Blue-violet light induced the negative phototropism prominently while red light had no effect.(3)The negative phototropism curvature could be promoted by exogenous Ca Cl2,but inhibited by exogenous La Cl3,meanwhile,exogenous Ca Cl2 could relieve the inhibiting effect of La Cl3.(4)Unilateral light induced horizontal transport of IAA from the irradiated side to the shaded side which resulting in the unequal distribution of IAA in the both sides that led to the formation of negative phototropism. The horizontal transporting of IAA was promoted exogenous Ca2+ but inhibited by exogenous La3+.

关 键 词：吊兰 根 水培 负向光性 机理 调控 

分 类 号：S682.36[农业科学—观赏园艺]