白鹤芋胚性细胞悬浮培养和高效植株再生体系的建立  

An Efficient System of Embryogenic Cell Suspension Cultures and Plant Regeneration in Spathiphyllum cannifolium

作　　者：于波[1] 刘金梅[1] 刘晓荣[1] 刘小飞[1] 廖飞雄[1] 

机构地区：[1]广东省农业科学院环境园艺研究所广东省园林花卉种质创新综合利用重点实验室,广州510640

出　　处：《园艺学报》2015年第4期721-730,共10页Acta Horticulturae Sinica

基　　金：广东省国际合作项目(2010B050600011;2011B050500010)

摘　　要：以白鹤芋(Spathiphyllum cannifolium)试管苗叶柄为外植体诱导获得胚性愈伤组织,建立了胚性细胞悬浮培养系并高频率再生出植株。结果表明,最优的悬浮培养条件为:装有20 m L液体培养基的100 m L三角瓶中接种0.3 g胚性细胞团,悬浮培养基为MS+0.5 mg·L-1 TDZ+1.0 mg·L-1 2,4-D+30g·L-1蔗糖或麦芽糖,p H 5.8;继代间隔14 d;每个继代周期,胚性细胞团可增殖至接种量的5倍以上;植株再生最优的分化培养基为1/2MS+0.3 mg·L-1 6-BA+30 g·L-1蔗糖+8.0 g·L-1琼脂粉,p H 5.8,平均每个胚性细胞团可分化再生出25.1株小植株;胚性细胞的快速增殖和高频率植株再生的状态可保持24周。This study established embryogenic cell suspension cultures of Spathiphyllum cannifolium through embryogenic calli induced from the petioles of plantlets in vitro,and further achieved high frequency of plant regeneration using the obtained suspension cultures. The results suggested that the optimal conditions of embryogenic cell suspension culture included:Inoculum amount was 0.3 g of embryogenic cell aggregates loaded with 20 m L fluid nutrient medium;Suspension culture medium was MS medium supplemented with 0.5 mg · L-1 N-phenyl-N'-1,2,3-thiadiazol-5-ylurea(TDZ),1.0 mg · L-1 2,4- Dichlorophenoxyacetic acid(2,4-D),and 30 g · L-1 sucrose or maltose(p H 5.8);Subculture interval was 14 days. Under these circumstances,the proliferation rate of embryogenic cell aggregates was 5-fold per subculture cycle. For plant regeneration,moreover,25.1 plantlets in average developed(or generated)from each embryogenic cell aggregate on the optimal differential medium of 1/2MS + 0.3 mg · L-1 6-BA + 30 g · L-1 sucrose + 8.0 g · L-1agar(p H 5.8). The status of rapid proliferation of embryogenic cells and efficient regeneration of plantlets could maintain 24 weeks.

关 键 词：天南星科 白鹤芋 胚性愈伤组织 液体培养 再生 

分 类 号：S682.36[农业科学—观赏园艺]