番茄抗叶霉病基因Cf19的抗性特点及遗传连锁分析  

Characteristic and Genetic Linkage Analysis of Cf19 Gene in Tomato

作　　者：赵婷婷[1] 张帆[1] 李帅[1] 刘冠[1] 李景富[1] 姜景彬[1] 张贺[1] 许向阳[1] 

机构地区：[1]东北农业大学园艺学院番茄研究所,哈尔滨150030

出　　处：《园艺学报》2015年第5期872-878,共7页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-25-A-15);国家自然科学基金项目(31272171);黑龙江省杰出青年科学基金项目(JC201204)

摘　　要：番茄抗叶霉病基因Cf19的抗性范围基本涵盖了中国目前分化出的所有叶霉菌生理小种,且抗性显著;台盼蓝染色结果显示该基因介导的抗性应答中出现明显的超敏反应,强度介于Cf4和Cf9基因之间;遗传连锁分析说明该基因的遗传特性符合单基因显性遗传规律;通过进一步SSR和AFLP分子标记连锁分析,共找到6个与Cf19基因连锁的分子标记,并将该基因初步定位于番茄1号染色体短臂区域。In this study,comprehensive research including resistance characteristic and genetic linkage analysis of Cf19 gene were carried out. Artificial inoculation results showed that tomato plants carrying Cf19 gene resistant to all physiological races in China. A remarkable hypersensitive response(HR)phenomenon was observed in trypan blue staining experiment,and the response intensity that Cf19 mediated is between Cf4 and Cf9. Inheritance analysis indicated that the genetic action of Cf19 fits the genetic pattern of a single dominant gene. Six markers linked to Cf19 gene were obtained in SSR and AFLP analysis,and of which the marker SSR336 located Cf19 gene on the short arm of chromosome 1.

关 键 词：番茄 番茄叶霉病 Cf19基因 遗传规律 基因定位 

分 类 号：S641.2[农业科学—蔬菜学]