核用银杏品种遗传关系的AFLP分析  

Genetic Relationship of Stone-used Ginkgo biloba Cultivars Based on AFLP Markers

作　　者：吴岐奎[1] 邢世岩[1] 王萱[1] 孙立民[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018

出　　处：《园艺学报》2015年第5期961-968,共8页Acta Horticulturae Sinica

基　　金：国家科技支撑计划课题(2012BAD21B04);山东省农业良种工程重大课题(鲁农良字[2011]7号);国家植物种质资源共享平台项目(2013-39)

摘　　要：采用AFLP技术对34份核用银杏品种进行遗传多样性和亲缘关系分析。结果表明:8对AFLP引物组合共扩增出1 337条谱带,多态带百分率为99.63%;品种间的遗传相似系数在0.2915~0.6812之间,平均值为0.4911;在相似系数为0.50时供试品种可分为4大类;依据AFLP结果建立了核用银杏品种指纹图谱。核用银杏4个类型的Nei’s遗传多样性指数范围为0.1392~0.2014,总体为0.2112,Shannon’s信息指数范围为0.2138~0.3165,总体为0.3417;4个类型品种间的遗传分化系数为0.0871,基因流为5.4230。结合特异位点、相似系数、聚类结果等筛选出8份核用银杏优质品种。The genetic relationship of 34 stone-used Ginkgo biloba L. cultivars were assessed by AFLP markers. A total of 1 337 bands were amplified with 8 pairs of primers,the percentage of polymorphic loci was 99.63%. The genetic similarity coefficient among 34 stone-used G. biloba L. cultivars ranged from 0.2915 to 0.6812 with an average of 0.4911,and all cultivars were divided into 4 groups at 0.50 of the similarity coefficient. The fingerprint of G. biloba L. cultivars were established based on AFLP results. Nei's gene diversity index ranged from 0.1392 to 0.2014 for 4 types of stone-used G. biloba L. cultivars,with the total value of 0.2112. Shannon's information index varied from 0.2138 to 0.3165,with the total value of 0.3417. The genetic differentiation coefficient for 4 types of stone-used G. biloba L. cultivars was 0.0871 and genetic flow was 5.4230. Eight cultivars were recognized relatively more distinctive among 34 stone-used G. biloba L. cultivars.

关 键 词：核用银杏 AFLP 遗传多样性 亲缘关系 

分 类 号：S664.3[农业科学—果树学]