不同地理种群麦长管蚜3种次级共生菌检测与系统发育分析  

Detection and phylogenetic analysis of three facultative endosymbionts in grain aphid,Sitobion avenaefrom different geographical regions

作　　者：罗晨[1] 胡祖庆[1] 于永昂[2] 杨杰[1] 张改生[2] 赵惠燕[1] 

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室植物保护学院,陕西杨凌712100 [2]西北农林科技大学国家杨凌农业生物技术育种中心,国家小麦改良中心杨凌分中心,小麦育种教育部工程研究中心,陕西省作物杂种优势研究与利用重点实验室,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2015年第1期141-146,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省科学技术研究发展计划项目(2013K02-12);西北农林科技大学基本科研业务费专项(QN2013011);西北农林科技大学博士后科研启动基金项目(2013BSJJ114)

摘　　要：【目的】次级共生菌Serratic symbiotica(PASS)、Hamiltonella defensa(PABS)、Regiella insecticola(PAUS)广泛存在于蚜虫体内。研究其在我国麦长管蚜(Sitobion avenae(Fabricius))体内的感染情况及遗传多样性,为进一步研究感染次级共生菌对麦长管蚜的生态学和生物学影响奠定基础。【方法】利用16SrDNA特异引物,对陕西杨凌、安徽滁州、陕西五丁关、河南郑州、山西太谷和新疆石河子6个地理种群麦长管蚜体内PASS、PABS和PAUS共生菌进行了Long-PCR检测、测序和系统发育分析。【结果】(1)所有6个地理种群均感染PASS和PAUS(感染率均为100%),而陕西杨凌、安徽滁州、新疆石河子3个地理种群感染PABS(感染率15%~70%),其余3个地理种群均未感染PABS。(2)16SrDNA序列的比对分析表明,感染PASS、PABS和PAUS的6个地理种群的株系均具有高度一致的序列。(3)基于16SrDNA序列构建的系统发育树表明,我国麦长管蚜体内感染的这3种次级共生菌中,PASS和PABS共生菌的亲缘关系较近。【结论】PASS和PAUS广泛存在于中国不同地理种群麦长管蚜体内,且具有较低的遗传多样性。【Objective】Secondary symbionts,Serratic symbiotica(PASS),Hamiltonella defensa(PABS)and Regiella insecticola(PAUS),exist widely in grain aphid,which induce changes in biological and ecological characteristics of aphids.This paper researched the genetic diversity and phylogeny of PASS,PABS and PAUS in 6geographical populations of Sitobion avenae in wheat in China.【Method】Special primers of 16 SrDNA were used to conduct Long-PCR detection,sequence analysis,and phylogenetic a-nalysis for PASS,PABS and PAUS in 6geographical regions including Yangling Shaanxi,Chuzhou Anhui,Wudingguan Shaanxi,Zhengzhou Henan,Taigu Shanxi,and Shihezi Xinjiang.【Result】(1)All 6geographical populations of S.avenae were infected by PASS and PAUS with the infection rate of 100%,and S.avenae populations from Yangling Shaanxi,Chuzhou Anhui,and Shihezi Xinjiang were also infected by PABS with the infection rate of 15%-70%.(2)The comparison of 16 SrDNA gene sequences showed that the gene sequences of PASS,PABS,and PAUS from all 6geographical populations were highly identical.(3)The phylogenetic tree constructed based on 16 SrDNA gene showed that PASS and PABS had close genetic relationship.【Conclusion】PASS and PAUS existed diffusely in S.avenae populations from different geographical regions of China,and they had poor genetic diversity.

关 键 词：麦长管蚜 地理种群 次级共生菌 系统发育分析 16SrDNA 

分 类 号：S433[农业科学—农业昆虫与害虫防治]