LH受体在雌性山羊肠系膜后神经节中的分布  

Distribution of LHR on caudal mesenteric ganglion of female goats

作　　者：董伟[1,2] 范洁 徐永平[1] 金秀芳[1] 郭瑜龙[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]畜牧兽医科学研究院,甘肃武威733000

出　　处：《西北农林科技大学学报（自然科学版）》2015年第2期23-27,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省自然科学基金项目(SJ08C105);国家自然科学基金项目(31072184)

摘　　要：【目的】探讨促黄体生成素(LH)是否可以通过影响雌性山羊肠系膜后神经节(CMG)参与动物机体生殖活动的调节。【方法】取5只未怀孕健康成年雌性山羊肠系膜后神经节,于多聚甲醛中固定,经石蜡包埋并制作厚度7μm的连续石蜡切片后,采用免疫组织化学SP染色方法观察雌性山羊CMG中LH受体(LHR)的分布特点,并计算LHR免疫反应阳性细胞的相对表达量。【结果】在CMG中,神经元细胞均为LHR免疫阳性反应,多数细胞呈中等阳性,少数细胞呈强阳性,LHR免疫阳性产物主要分布在神经元的细胞膜、细胞质和细胞核膜上,但细胞核为阴性反应。神经纤维和卫星细胞中的LHR免疫阳性产物为弱阳性表达。神经元细胞上LHR的相对表达量(7.057±0.797)极显著高于其他非神经元细胞(1.497±0.690)(P<0.01)。【结论】CMG中神经元细胞是LH发挥作用的主要靶细胞,提示CMG神经元细胞中存在的LHR可能是协调LH对生殖器官的内分泌调节和自主神经对生殖器官的神经调节的中转站。【Objective】This study aimed to investigate whether LH could regulate reproductive activity through the effect on caudal mesenteric ganglion(CMG)of female goat.【Method】Caudal mesenteric ganglions were taken from five healthy adult female goats.The caudal mesenteric ganglions were made into 7μm serial paraffin section after fixed by paraformaldehyde and embedded by paraffin.Immunohistochemical SP method was adopted to observe the distribution of LH receptor(LHR)in caudal mesenteric ganglion of female goats.【Result】All the neurons in CMG responded positively to LHR,and most of these substances showed moderately positive expression while minor were strong positive.LHR immunoreactive substances mainly distributed in membrane and cytoplasm of neurons,but the nucleus were negative to LHR.However,in supporting cells and nerve fibers,weakly positive expression was observed.Significantly higher relative expression of LHR was observed in neurons(7.057±0.797)compared to that in other non-nerve cells(1.497±0.690)(P<0.01).【Conclusion】Neurons in caudal mesenteric ganglion were the main target cells of LH,indicating that LHR existing in these neurons might be a'transfer station'to coordinate the endocrine regulation and nervous regulation.

关 键 词：促黄体生成素受体 肠系膜后神经节 免疫组织化学SP法 雌性山羊 

分 类 号：S827[农业科学—畜牧学]