禁食后再投喂对厚颌鲂幼鱼生化及非特异性免疫指标的影响  

Effects of fasting and re-feeding on biochemical and non-specific immune indexes of Megalobrama pellegrini juvenile

作　　者：李芹[1] 唐洪玉[1] 郑永华[1] 邓丽蓉[1] 

机构地区：[1]西南大学动物科技学院水产科学系,重庆北碚400715

出　　处：《西北农林科技大学学报（自然科学版）》2015年第2期49-57,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：中央高校基本科研业务费专项(XDJK2012C091);中国长江三峡集团公司项目;农业部科技教育司项目(200903048-08)

摘　　要：【目的】研究禁食后再投喂对厚颌鲂幼鱼生长、血液学、生物化学及非特异性免疫指标的影响。【方法】将厚颌鲂幼鱼分成4组,分别为对照组(正常投喂24d,F0)、禁食3d后连续投喂21d(F3组)、禁食7d后连续投喂17d(F7组)、禁食12d后连续投喂12d(F12组),在禁食前、禁食后及恢复投喂后取样,检测其生长指标(体质量、体长、肥满度、肝体指数(HSI)、脏体指数(VSI))、红细胞压积、血清皮质醇质量浓度、血糖浓度及血清、肝胰脏和头肾超氧化物歧化酶(SOD)、溶菌酶活性。【结果】禁食后,厚颌鲂幼鱼的肥满度、肝体指数、脏体指数大多显著降低(P<0.05),恢复投喂后均能恢复至正常水平(F12组VSI除外)。禁食后恢复投喂对幼鱼的红细胞压积无显著影响(P>0.05)。F3、F7组幼鱼的血糖在禁食后与对照组(F0)无显著性差异(P>0.05),而F12组幼鱼在禁食后血糖浓度显著下降(P<0.05),恢复投喂后各试验组血糖浓度均恢复至正常水平。禁食后F3组幼鱼皮质醇质量浓度与对照组(F0)无显著性差异(P>0.05),而F7、F12组幼鱼皮质醇质量浓度则极显著升高(P<0.01),恢复投喂后均恢复至对照组(F0)水平。禁食后F3组幼鱼血清和肝胰脏SOD活性与对照组(F0)无显著性差异(P>0.05),F7、F12组幼鱼血清和肝胰脏SOD活性显著降低(P<0.05),恢复投喂后血清和F3、F7组肝胰脏SOD活性与对照组无显著性差异(P>0.05),F12组幼鱼肝胰脏SOD活性显著低于对照组(P<0.05)。禁食后再投喂对厚颌鲂幼鱼肝胰脏溶菌酶活性无显著影响(P>0.05)。禁食后F3、F7组幼鱼血清溶菌酶活性与对照组(F0)无显著性差异(P>0.05),F12组幼鱼血清溶菌酶活性显著低于对照组(P<0.05),恢复投喂后,各试验组血清溶菌酶活性均与对照组(F0)无显著差异(P>0.05)。禁食后,F3组幼鱼头肾溶菌酶活性与对照组(F0)无显著性差异(P>0.05),F7、F12组幼鱼头肾溶菌酶活性显著降低(P<0.05),恢复投喂后,F【Objective】The effects of fasting and re-feeding on growth and hematological,biochemicaland non-specific immune indexes of Megalobrama pellegrini juvenile were studied.【Method】Megalobrama pellegrini juveniles were distributed in four experimental groups.The first group was considered as the control(fed normally F0)while the other three groups were fasted for 3d(F3),7d(F7),and 12d(F12),respectively before re-feeding to 24 d.The growth parameters(weight,length,fatness K,HSI,and VSI),hematocrit,serum cortisol,blood glucose and serum,hepatopancreas,head-kidney SOD activities and lysozyme activities in Megalobrama pellegrini juveniles were determined before and after fasting and after refeeding.【Result】After fasting,most of the growth parameters including fatness K,HIS,and VSI significantly decreased(P<0.05),and these parameters could return to normal levels after re-feeding(except for the VSI of F12).The hematocrit of juveniles was not significantly affected by fasting and re-feeding(P>0.05).Blood glucose values of juveniles in F3 and F7remained unchanged during fasting with no significantly difference compared with the control(P>0.05),but blood glucose values of juveniles in F12 significantly decreased(P<0.05).After re-feeding,the blood glucose of juveniles could return to the same levels as the control.After fasting,the cortisol levels of F3 were not significantly different(P>0.05).Fasting periods of7 dand 12dsignificantly increased serum cortisol levels compared with the control(P<0.01),and they returned to basal levels after re-feeding.After fasting,the serum and hepatopancreas SOD activities of F3 were not significantly different(P>0.05),while the SOD activities of F7 and F12significantly decreased(P<0.05).After re-feeding,the serum SOD activities and hepatopancreas SOD activities of F3 and F7group returned to the levels of the control group,but the hepatopancreas SOD activities of F12 remained significantly lower than that of the control(P<0.05).The hepatopancreas lysozyme activities of juveniles we

关 键 词：厚颌鲂(Megalobrama pellegrini) 禁食-再投喂 生长指标 生物化学指标 非特异性免疫指标 

分 类 号：S917.4[农业科学—水产科学]