甘南牦牛CYGB基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of CYGB gene of Gannan yak

作　　者：娄延岳 杜晓华[3,2] 罗玉柱[2] 刘霞[1,2] 张磊[4] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃省草食动物生物技术重点实验室,甘肃兰州730070 [3]甘肃农业大学动物医学院,甘肃兰州730070 [4]河南农业职业学院 动物科学系,河南郑州451450

出　　处：《西北农林科技大学学报（自然科学版）》2015年第4期1-6,11,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：甘肃省自然科学基金项目(1107RJZA148);甘肃省教育厅研究生导师项目(1102-04);甘肃省高等学校基本科研业务费资助项目(2013);甘肃农业大学科技创新基金项目(GAU-CX1037;GAU-CX1038)

摘　　要：【目的】探讨甘南牦牛CYGB基因的分子结构特征及生理功能。【方法】提取甘南牦牛血样基因组DNA,以其为模板PCR分段扩增后拼接得到CYGB基因,测序后进行生物信息学分析。【结果】克隆出甘南牦牛CYGB基因,其长度为8 484bp,含有4个外显子和3个内含子,外显子长度分别为143,232,164和34bp,内含子长度分别为5 175,280和2 379bp,可编码190个氨基酸残基,氨基酸编码存在明显的密码子偏倚现象。与普通牛相比较,甘南牦牛CYGB基因存在73处核苷酸变异位点,其中2处发生在外显子2区域,氨基酸序列未发生改变;71处发生在内含子区域。甘南牦牛CYGB拥有"three-over-three"类型的螺旋"三明治"折叠结构。系统发育树分析表明,甘南牦牛CYGB与黄牛、绵羊等物种之间存在较高的同源性。【结论】成功克隆了甘南牦牛CYGB基因,为进一步研究牦牛CYGB的遗传特性和生理机制奠定了基础。【Objective】This study aimed to elucidate the structure and physiological function of CYGB gene.【Method】The CYGBgene of Gannan yak was amplified through PCR using extracted genomic DNA sequence from blood samples as template.Then the gene was sequenced and analyzed by bioinformatics tools.【Result】The length of cloned Gannan yak CYGB gene was 8 484 bp,including four exons with lengths of 143,232,164 and 34bp,and three introns with lengths of 5 175,280 and 2 379 bp,respectively.A total of 190 amino acids were encoded and the usage of synonymous codons was biased.Compared with taurine cattle(Bos taurus),there were 73 nucleotide mutations in CYGBgene of Gannan yak,among which2 occurred in exon region and 71 occurred in intron region.There were no changes in amino acids.Gannan yak CYGBgene had a spiral sandwich like folded structure with the'three-over-three'type.Phylogenetic analysis showed that Gannan yak CYGBgene had a high homology with Bos taurus,Ovis aries and other species.【Conclusion】Gannan yak CYGBgene was successfully cloned and could be used for further study on genetic characteristics and physiological mechanism.

关 键 词：甘南牦牛 胞红蛋白 基因 生物信息学 

分 类 号：S823.85[农业科学—畜牧学]