纳米羟基磷灰石负载埃博霉素B的细胞活性研究  被引量:2

Antitumor Activity of Epothilone B Attached on Nano-Hydroxyapatite Surface

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作  者:张露露[1] 张松[1] 孙瑞成[1] 刘新利[1] 

机构地区:[1]齐鲁工业大学生物工程学院,济南250353

出  处:《化学通报》2015年第1期89-92,共4页Chemistry

基  金:山东省科技发展计划项目(2012GSF12107)资助

摘  要:羟基磷灰石(HA)纳米材料因其生物相容性好,比表面积大,可以吸附大量的药物分子,因此在纳米医药领域极具应用前景。本文通过共沉淀法制备出纳米HA(HA NPs),然后利用XRD,FT IR,SEM,TEM进行表征,结果表明,所制备的HA NPs呈针形,长约70~90nm。实验表明,采用二氯甲烷作为溶剂,埃博霉素B(Epo B)负载效率最高,最大负载量达0.53g/(g HA)。应用MTT法对HA NPs及复合物的抗肿瘤活性进行分析,结果显示HA-Epo B复合物对肺癌细胞NCI-H292和LLC的IC50浓度分别为15和35 ng/m L,明显低于游离Epo B的IC5035和50 ng/m L。这可能是HA NPs更容易进入细胞内,然后将药物释放,进而抑制肿瘤细胞生长。Hydroxyapatite( HA) materials have shown applications in biomedicine due to their high biocompatibility and large surface area. Hydroxyapatite nanoparticles( HA NPs) were synthesized by co-precipitation and characterized by XRD,FT IR,SEM and TEM. The results showed that HA NPs are in shape of needles with a length of 70 ~ 90 nm. The maximum carrying capacity,0. 53 g / g HA,was obtained in methylene chloride for Epothilone B( Epo B). The results of the MTT assay indicated that the IC50 values for HA-Epo B on NCI-H292 and LLC are 15 and 35 ng / m L respectively,which are less than that for Epo B itself. This means that the HA NPs can enter the cells easily,release the drugs in intracellular,and then inhibit the growing of cancer cells.

关 键 词:羟基磷灰石纳米颗粒 EPOTHILONE B 药物载体 肿瘤细胞 抗肿瘤活性 

分 类 号:TQ460.1[化学工程—制药化工]

 

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