自组装短肽GFS-2构建新型三维细胞培养支架材料  

Designer Self-Assembling Peptide GFS-2 Nanofiber Scaffolds for 3D Cell Cultures

作　　者：岳媛媛[1] 李萌萌[1] 徐晓帆[1] 罗忠礼[1] 

机构地区：[1]重庆医科大学基础医学院分子与肿瘤医学研究中心,重庆400016

出　　处：《化学通报》2015年第3期273-276,共4页Chemistry

基　　金：国家自然科学基金项目(81101417);高等学校博士学科点专项科研基金项目(20115503120010);重庆市自然科学基金项目(2011bb5134)资助

摘　　要：典型的自组装短肽在水中可形成稳定的β-sheet二级结构,在生理性环境下则能够形成稳定的纳米纤维,可进一步形成含水量达99%的水凝胶,具有高纯度、可降解及无免疫反应等突出优点,能模拟生物体内的三维基质环境而作为细胞三维培养的新型生物材料。本文主要采用了圆二色谱仪、原子力显微镜、倒置显微镜等探究新型短肽GFS-2的自组装性能及其在细胞三维培养中的应用。此研究可能会启发设计更多的新型自组装短肽服务于化学、生物材料、医学工程等领域。The typical self-assembly peptide has a stable β-sheet secondary structure in aqueous solution. They can form ordered-nanofiber in physiological solution,and the self-assembled hydrogels has extremely high water content( up to 99%). With competitive advantages,like high purity,biodegradable and no immunological reaction,this kind of hydrogel with matrix can mimic 3-dimensional( 3D) microenvironment in the body and can be used as a3 D cell culture biomaterial. We investigated a new type self-assembly peptide named GFS-2 by circular dichroism spectroscopy and atomic force microscopy, inverted microscope and 3D Cell culture, confirmed its secondary structure,properties and application. Our study could inspire more peptide designed for chemical,biomaterials,and biomedical engineering and beyond.

关 键 词：自组装短肽 β-sheet二级结构 纳米纤维 水凝胶 三维细胞培养 

分 类 号：TQ342.8[化学工程—化纤工业] Q813.11[生物学—生物工程]